Anti-LIMK1 (Thr-508) [LIMK2 (Thr-505], Phosphospecific Antibody

Name: Anti-LIMK1 (Thr-508) [LIMK2 (Thr-505], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: LP1891
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

1 Citation

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

LIMK1

Molecular Weight

Go to Full Product Details

Western blot image of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-term.) (lanes 1 & 2) and anti-LIMK1 (Thr-508) (lanes 3 & 4).

Volume: 100 µL

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$309

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Product Details

Target

LIMK1 (Thr-508)[LIMK2 (Thr-505]

Target Description

LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. In addition, LIMKs may have a function in the nucleus. It has been shown that the nuclear localization of LIMKs can mediate suppression of Rac/Cdc42-mediated cyclin D1 expression. This effect of LIMKs was independent of cofilin phosphorylation and the regulation of actin dynamics.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

LIMK1

Molecular Weight

Antigen

LIMK1 (Thr-508) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 508 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Thr-505 in human LIMK2.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 72 kDa* protein corresponding to the molecular mass of LIMK1 on SDS-PAGE immunoblots of activated mouse recombinant LIMK1, but does not detect this protein after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-508

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

LIMK

UniProt Details

UniProt (Human): P53667
UniProt (Immunogen Species): P53667

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
35318161	not listed	Meyers, CMG, et al. 2022. Histone Deacetylase 5 is a Phosphorylation Substrate of Protein Kinase D in Osteoclasts. Bone, 116393.

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Anti-LIMK1 (Thr-508) [LIMK2 (Thr-505], Phosphospecific Antibody