Name: Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) Antibody
Brand: PhosphoSolutions
SKU: p170-5359
Price: 415.0 USD
Availability: InStock

Our Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Interferon-α Receptor, Type I, Subunit I (Ser535,539) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Rat, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

IFNAR1

Molecular Weight

110-130 kDa

Go to Full Product Details

Western blot of immunoprecipitates from HEK 293 cells transfected with 1) Mock, 2) IFNAR1 WT, and 3) IFNAR1 S535A and S539A mutants. Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) Antibody

Product Details

Target

Interferon-α Receptor, Type I, Subunit I (Ser535,539)

Target Description

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine-535 and Ser-539 in the IFNAR1 (Kumar et al., 2003).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

IFNAR1

Molecular Weight

110-130 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Rat, Sheep

Antibody Registry ID

AB_2492138

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 kDa - 130 kDa.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser535,539

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Alpha-Type Antiviral Protein, AVP, Beta-Type Antiviral Protein, CRF2-1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, IFN-Alpha/Beta Receptor 1, IFNBR, IFN-Alpha-REC, IFNAR, IFN-R-1, IFRC, Interferon Alpha/Beta Receptor 1, Interferon-Alpha/Beta Receptor Alpha Chain, Interferon Alpha And Beta Receptor Subunit 1, Interferon (Alpha, Beta And Omega) Receptor 1, Interferon-Beta Receptor 1, Interferon Receptor 1, Type I Interferon Receptor 1

UniProt Details

UniProt (Human): P17181
UniProt (Immunogen Species): P17181

NCBI Gene ID

3654

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
21865166	not listed	Zheng, H., et al. 2011. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. Journal of Biological Chemistry, 286(41), pp.35733-35741.
21695243	not listed	Qian, J., et al. 2011. Pathogen recognition receptor signaling accelerates phosphorylation-dependent degradation of IFNAR1. PLoS Pathog, 7(6), p.e1002065.
21540188	not listed	Bhattacharya, S., et al. 2011. Role of p38 protein kinase in the ligand-independent ubiquitination and down-regulation of the IFNAR1 chain of type I interferon receptor. Journal of Biological Chemistry, 286(25), pp.22069-22076.
19948722	not listed	Bhattacharya, S., et al. 2010. Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor. J Biol Chem. 285(4):2318-25.
19805514	not listed	Liu, J., et al. 2009. Mammalian casein kinase 1α and its leishmanial ortholog regulate stability of IFNAR1 and type I interferon signaling. Molecular and cellular biology, 29(24), pp.6401-6412.
15337770	not listed	Kumar, K.G., et al. 2004. Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 - 46620.

Western Blot: Human
PMID	Dilution	Publication
21695243	not listed	Qian, J., et al. 2011. Pathogen recognition receptor signaling accelerates phosphorylation-dependent degradation of IFNAR1. PLoS Pathog, 7(6), p.e1002065.
21540188	not listed	Bhattacharya, S., et al. 2011. Role of p38 protein kinase in the ligand-independent ubiquitination and down-regulation of the IFNAR1 chain of type I interferon receptor. Journal of Biological Chemistry, 286(25), pp.22069-22076.
19805514	not listed	Liu, J., et al. 2009. Mammalian casein kinase 1α and its leishmanial ortholog regulate stability of IFNAR1 and type I interferon signaling. Molecular and cellular biology, 29(24), pp.6401-6412.

Product Specific Protocols

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Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) Antibody