Anti-Integrin β4 (Tyr-1526), Phosphospecific Antibody

Name: Anti-Integrin β4 (Tyr-1526), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: IP1291
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

ITGB4

Molecular Weight

200

Go to Full Product Details

Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).

Volume: 100 µL

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Price:

$309

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Bulk Order Anti-Integrin β4 (Tyr-1526), Phosphospecific Antibody

Product Details

Target

Integrin β4 (Tyr-1526)

Target Description

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

ITGB4

Molecular Weight

200

Antigen

Phospho-Integrin β4 (Tyr-1526) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1526 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to unphosphorylated Integrin β4 (Tyr-1526) peptide before affinity purification using phospho-Integrin β4 (Tyr-1526) peptide (without carrier). The purified antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of A431 and src-transformed Hct116 cells treated with pervanadate, but not in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-1526

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

integrin, CD104, GP150

UniProt Details

UniProt (Human): P16144
UniProt (Immunogen Species): P16144

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Integrin β4 (Tyr-1526), Phosphospecific Antibody