Our Integrin β1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Integrin β1 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.
Western blot analysis of native cell lysates from human NCI-H446 lung cancer cells (lane 1), LNCaP prostate cancer cells (lane 2), and MCF7 breast cancer cells (lane 3). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0411) at 1:1000 dilution.
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Protein G Purified
Monoclonal
M041
IgG2a
ICC, IP, WB
Mouse
ITGB1
120-150
Clone M041 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the NCI-H1915 lung cancer cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
Clone M0411 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, LNCaP, MCF7, and NCI-H446 cell lysates, as well as human lung tissue. The antibody does not detect the denatured form of Integrin β1. Clone M0411 can be used in western blot, immunocytochemistry, and immunoprecipitation, as well as for ELISA detection when paired with Integrin β1 IM0041 as the capture antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
