Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and LNCaP (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0041) at 1:1000 dilution.
Anti-Integrin β1 (Extracellular region) Antibody
SKU: IM0041
Bulk Order Anti-Integrin β1 (Extracellular region) Antibody
Product Details
Integrin β1 (Extracellular region)
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Protein G Purified
Monoclonal
M004
IgG1
ICC, IP, WB
Mouse
ITGB1
120-150
Clone M004 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the A431 epidermoid carcinoma cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
Clone M004 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, A549, MDA-MB-231, and LNCaP cell lysates. The antibody also detects native recombinant human Integrin α1/β1, α2/β1, and α3/β1 complexes, but does not detect recombinant human Integrin α6/β4 or αV/β3 complexes. The antibody does not detect the denatured form of Integrin β1. Clone M004 can be used in western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well for detecting live, unfixed cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
