Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody (39A1413)

Name: Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: IM3741
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

39A1413

Applications

IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

NFKBIA

Molecular Weight

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Western blot analysis of Jurkat cells untreated (lanes 1 & 3) or treated with TNFα (1 nM). The blots were probed with anti-IκBα (lanes 1 & 2) or anti-IκBα (Ser-32/Ser-36) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$255

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Bulk Order Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody

Product Details

Target

IκBα (Ser-32/Ser-36)

Target Description

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Format

Protein A Purified

Clonality

Monoclonal

Clone

39A1413

Isotype

IgG1

Applications

IP, WB

Host Species

Mouse

Gene Name

NFKBIA

Molecular Weight

Antigen

Clone 39A1413 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 32 and 36 of human IκBα. This peptide sequence is highly conserved in mouse, rat, dog, cow, and pig IκBα.

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.5% BSA and 0.05% NaN3

Dilution Ranges

WB: 1:500
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of Jurkat cells treated with calpain inhibitor (ALLN) followed by TNFα, but the antibody does not detect this band in untreated cells.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa

UniProt Details

UniProt (Human): P25963
UniProt (Immunogen Species): P25963

Product Specific Protocols

Western Blot Protocol: Download

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Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody (39A1413)

Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody
SKU: IM3741

Bulk Order Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody

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Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody (39A1413)

Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody SKU: IM3741

Bulk Order Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody

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Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody
SKU: IM3741