Our Anti-His (C-terminal) Tag Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects na Anti-His (C-terminal) Tag Antibody and is Protein G purified. It is great for use in WB, ICC, IHC, IP, FC.
Western blot of human recombinant DDR1 protein with C-terminal His Tag. The blot was probed with mouse monoclonal anti-His (Cterminal) Tag (HM0501) at 1:2000 (lane 1), 1:4000 (lane 2), and 1:8000 (lane 3).
Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays.
Protein G Purified
Monoclonal
M050
IgG1
ICC, IF, IHC, IP, WB
Mouse
N/A
N/A
Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:5000
IHC: 1:100
ICC: 1:100
IP: 1:100
Unconjugated
Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunoprecipitation, immunocytochemistry, flow cytometry, and ELISA capture.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
His Tag, 6x His Tag, HHHHHH
UniProt (Immunogen Species): N/A
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