Western blot. Left Lane -- Cultured COS cells were transfected with a plasmid containing the SAP1a cDNA fused with an HA epitope tag at its C-terminus. Right Lane -- COS transfected with an empty plasmid vector. 25 ug of protein loaded in each lane.
Anti-HA Epitope Tag Antibody
HA Epitope Tag Antibody
ELISA, ICC, IHC, IP, WB
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).
Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
You may also like
We stand by what we sell.
We are proud of every product that leaves our lab.
Support from the source.
No one knows our products like us, and we want you to succeed.