Anti-GSK-3β (N-terminal region) Antibody (M131)

Name: Anti-GSK-3β (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: GM1311
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M131

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

GSK3B

Molecular Weight

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Immunocytochemical labeling of GSK3β in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK3β (GM1311) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis of a human recombinant GSK3β N-terminal fragment (lanes 1 & 2) and endogenous GSK3β expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3β at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).

Volume: 100 µL

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Product Details

Target

GSK-3β (N-terminal region)

Target Description

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M131

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GSK3B

Molecular Weight

Antigen

Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3β. This sequence is highly conserved in rat and mouse GSK-3β, and has 65% homology to similar regions in GSK-3α.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IHC: 1:250
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of GSK-3β on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Glycogen synthase kinase beta3

UniProt Details

UniProt (Human): P49841
UniProt (Immunogen Species): P49841

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Anti-GSK-3β (N-terminal region) Antibody (M131)

Anti-GSK-3β (N-terminal region) Antibody
SKU: GM1311

Bulk Order Anti-GSK-3β (N-terminal region) Antibody

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Anti-GSK-3β (N-terminal region) Antibody (M131)

Anti-GSK-3β (N-terminal region) Antibody SKU: GM1311

Bulk Order Anti-GSK-3β (N-terminal region) Antibody

Product Details

Anti-GSK-3β (N-terminal region) Antibody
SKU: GM1311