Anti-GluN1/NR1 Glutamate Receptor Antibody (N308/48)
Detect GluN1/NR1 Glutamate Receptor with this antibody validated for IHC, WB, ICC.
Primary Antibody
Human, Mouse, Rat
N308/48
ICC, IHC, WB
Mouse
Purified
Grin1 Nmdar1
105 kDa
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-272-n308-48-wb2_{width}x.jpg?v=1626903458)
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with N308/48 (left) or N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-272-n308-48-wb2_1024x.jpg?v=1626903458)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Immunoprecipitation: Mouse
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 27746712 | 10 µg/mL | Bagasrawala, I., et al. 2016. N-Methyl D-Aspartate Receptor Antagonist Kynurenic Acid Affects Human Cortical Development. Frontiers in Neuroscience, 435. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34962957 | 1:200 | Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791. |
| 34781056 | not listed | Wang, B., et al. 2021. Lipid bilayers regulate allosteric signal of NMDA receptor GluN1 C-terminal domain. Biochemical and biophysical research communications, 15-21. |
| 33689678 | 1:200 | Fang, H., et al. 2021. An optimized CRISPR/Cas9 approach for precise genome editing in neurons. eLife, e65202. |
| 31554851 | not listed | Plumbly, W., et al. 2019. L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks. Scientific Reports, 13810. |
| 30333730 | 1:1000 | Zhou, L., et al. 2018. The C-terminus of NMDAR GluN1-1a subunit translocates to nucleus and regulates synaptic function. Frontiers in Cellular Neuroscience, 334. |
| 29760409 | 1:500 | Ma, K., et al. 2018. Histone deacetylase inhibitor MS-275 restores social and synaptic function in a Shank3-deficient mouse model of autism. Neuropsychopharmacology, 1779-1788. |
| 25644714 | not listed | DiBattista, A.M., et al. 2015. Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.. Biochimica et Biophysica Acta, 904-917. |
| 24032433 | 1:100 | Wang, X., et al. 2013. Activation of α7-containing nicotinic receptors on astrocytes triggers AMPA receptor recruitment to glutamatergic synapses. Journal of Neurochemistry, 632-643. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32604880 | not listed | García, F., et al. 2020. Astaxanthin Counteracts Excitotoxicity and Reduces the Ensuing Increases in Calcium Levels and Mitochondrial Reactive Oxygen Species Generation. Marine Drugs, 335. |
| 26675527 | not listed | Lee, N.J., et al. 2016. Hexa (ethylene glycol) derivative of benzothiazole aniline promotes dendritic spine formation through the RasGRF1-Ras dependent pathway.. Biochimica et Biophysica Acta, 284-295. |
| 24089484 | 1:500 | Duffney, L.J., et al. 2013. Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism.. The Journal of Neuroscience, 15767-15778. |
| 24012668 | not listed | YI, S., et al. 2013. Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking.. Biochemical and Biophysical Research Communications, . |
| 23774277 | 1:500 | Cheng, J., et al. 2013. SNARE proteins are essential in the potentiation of NMDA receptors by group II metabotropic glutamate receptors.. The Journal of Physiology, 3935-3947. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36609445 | 1:100 | Williams, J.B., et al. 2023. Inhibition of Histone Methyltransferase Smyd3 Rescues NMDAR and Cognitive Deficits in a Tauopathy Mouse Model. Nature Communications, 91. |
| 30054448 | 1:500 | Risher, W.C., et al. 2018. Thrombospondin receptor α2δ-1 promotes synaptogenesis and spinogenesis via postsynaptic Rac1. Journal of Cell Biology, 3747-3765. |
| 26317010 | 1:300 | Fourie, C., et al. 2014. Differential Changes in Postsynaptic Density Proteins in Postmortem Huntington''s Disease and Parkinson''s Disease Human Brains.. Journal of Neurodegenerative Diseases, 938530. |
| 25352748 | 1:500 | de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-1433. |
| 24336719 | 1:1000 | Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. The Journal of Neuroscience, 19534-19554. |
| 23233784 | 1:500 | DeParis, S.W., et al. 2012. Intrinsically photosensitive retinal ganglion cells are resistant to N-methyl-D-aspartic acid excitotoxicity.. Molecular Vision, 2814-2827. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 26930443 | 1:2000 | Rambousek, L., et al. 2016. Rat intra-hippocampal NMDA infusion induces cell-specific damage and changes in expression of NMDA and GABAA receptor subunits. Neuropharmacology, 594-606. |
| 25822337 | 5 µg/mL | Balsara, R., et al. 2015. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke.. PLoS One, e0122840. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 36609445 | 8ul | Williams, J.B., et al. 2023. Inhibition of Histone Methyltransferase Smyd3 Rescues NMDAR and Cognitive Deficits in a Tauopathy Mouse Model. Nature Communications, 91. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 30355732 | not listed | Zhou, J.J., et al. 2018. The α2δ-1–NMDA receptor coupling is essential for corticostriatal long-term potentiation and is involved in learning and memory. Journal of Biological Chemistry, 19354-19364. |
| 27746712 | 10 µg/mL | Bagasrawala, I., et al. 2016. N-Methyl D-Aspartate Receptor Antagonist Kynurenic Acid Affects Human Cortical Development. Frontiers in Neuroscience, 435. |
| 25635140 | 1:5000 | Xu, M., et al. 2015. Cysteine Substitution of Transmembrane Domain Amino Acids Alters the Ethanol Inhibition of GluN1/GluN2A N-Methyl-d-Aspartate Receptors. The Journal of Pharmacology and Experimental Therapeutics, 91-101. |
| 25316086 | 1:300 | Maraschi, A.M., et al. 2014. Parkin regulates kainate receptors by interacting with the GluK2 subunit.. Nature Communications, 5182. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36609445 | 1:500 | Williams, J.B., et al. 2023. Inhibition of Histone Methyltransferase Smyd3 Rescues NMDAR and Cognitive Deficits in a Tauopathy Mouse Model. Nature Communications, 91. |
| 36421705 | 1:500-1:1500 | Maisterrena, A., et al. 2022. The State Of The Dopaminergic And Glutamatergic Systems In The Valproic Acid Mouse Model Of Autism Spectrum Disorder. Biomolecules, . |
| 35789851 | 1:1000 | Huo, Y., et al. 2022. Prkn Knockout Mice Show Autistic-like Behaviors and Aberrant Synapse Formation. iScience, 104573. |
| 34962957 | 1:1000 | Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791. |
| 33542189 | 1:500 | Zhang, F., et al. 2021. Synergistic inhibition of histone modifiers produces therapeutic effects in adult Shank3-deficient mice. Translational Psychiatry, 99. |
| 33402706 | not listed | Mossa, A., et al. 2021. Developmental impaired Akt signaling in the Shank1 and Shank3 double knock-out mice. Molecular Psychiatry, 1928–1944. |
| 33298440 | 1:1000 | Cao, Q., et al. 2020. Targeting histone K4 trimethylation fortreatment of cognitive and synaptic deficits in mouse models of Alzheimer’s disease. Science Advances, eabc8096. |
| 33229412 | not listed | Pandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, ENEURO.0284-20.2020. |
| 33158444 | 1:1000 | Espinoza, S., et al. 2020. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biology, 164. |
| 31200742 | 1:1000 | Micci, M.A., et al. 2019. Hippocampal stem cells promotes synaptic resistance to the dysfunctional impact of amyloid beta oligomers via secreted exosomes. Molecular Neurodegeneration, 25. |
| 29706865 | 1:1000 | Khlghatyan, J., et al. 2018. Mental illnesses-associated Fxr1 and its negative regulator Gsk3β are modulators of anxiety and glutamatergic neurotransmission. Frontiers in Molecular Neuroscience, 119. |
| 29069439 | 1:100 | Shahin, S., et al. 2018. From the cover: 2.45-GHz microwave radiation impairs hippocampal learning and spatial memory: Involvement of local stress mechanism-induced suppression of …. Toxicological Sciences, 349-374. |
| 28215989 | not listed | Li, Q., et al. 2017. Klotho regulates CA1 hippocampal synaptic plasticity. Neuroscience, 123-133. |
| 27475724 | 1:500 | Qin, L., et al. 2016. The ADHD-linked human dopamine D4 receptor variant D4. 7 induces over-suppression of NMDA receptor function in prefrontal cortex. Neurobiology of Disease, 194-203. |
| 27334260 | not listed | Huang, L., et al. 2016. Post-anesthesia AMPA receptor potentiation prevents anesthesia-induced learning and synaptic deficits. Science Translational Medicine, 344ra85. |
| 27098698 | 1:10 | D'Orsi, B., et al. 2016. Bok is not pro-apoptotic but suppresses poly ADP-ribose polymerase-dependent cell death pathways and protects against excitotoxic and seizure-induced neuronal …. The Journal of Neuroscience, 4564-4578. |
| 27066908 | not listed | Vingtdeux, V., et al. 2016. CALHM1 deficiency impairs cerebral neuron activity and memory flexibility in mice. Scientific Reports, 24250. |
| 26714288 | not listed | Moraga-Amaro, R., et al. 2016. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.. Neuropharmacology, 222-235. |
| 26306919 | 1:200 | Lv, J.M., et al. 2016. The Noncompetitive AMPAR Antagonist Perampanel Abrogates Brain Endothelial Cell Permeability in Response to Ischemia: Involvement of Claudin-5.. Cellular and Molecular Neurobiology, 745-753. |
| 25644714 | not listed | DiBattista, A.M., et al. 2015. Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.. Biochimica et Biophysica Acta, 904-917. |
| 25632145 | 1:10 | D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. The Journal of Neuroscience, 1706-1722. |
| 25471585 | 1:500 | Warmus, B.A., et al. 2014. Tau-mediated NMDA receptor impairment underlies dysfunction of a selectively vulnerable network in a mouse model of frontotemporal dementia.. The Journal of Neuroscience, 16482-16495. |
| 25407677 | 1:1000 | Gomez, A.M., et al. 2014. Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.. eLife, e04287. |
| 25308431 | 1:1000 | Carney, K.E., et al. 2014. Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins.. Journal of Proteome Research, 5918-5927. |
| 25146373 | not listed | Yan, Y., et al. 2015. Kalirin-9 and Kalirin-12 Play Essential Roles in Dendritic Outgrowth and Branching.. Cerebral Cortex, 3487-3501. |
| 24094580 | 1:500 | Deng, Y., et al. 2014. β-amyloid impairs the regulation of N-methyl-D-aspartate receptors by glycogen synthase kinase 3.. Neurobiology of Aging, 449-459. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 35862168 | 1:500 | Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360. |
| 33593893 | not listed | Buss, E.W., et al. 2021. Cognitive aging is associated with redistribution of synaptic weights in the hippocampus. PNAS, e1921481118. |
| 29487522 | 1:1000 | Uttl, L., et al. 2018. Chronic MK-801 application in adolescence and early adulthood: a spatial working memory deficit in adult Long-Evans rats but no changes in the …. Frontiers in Pharmacology, 42. |
| 27147118 | 1:3000 | Spencer, K.B., et al. 2016. FMRP mediates chronic ethanol‐induced changes in NMDA, Kv4. 2, and KChIP3 expression in the hippocampus. . Alcoholism: Clinical and Experimental Research, 1251-1261. |
| 24103031 | 1:500 | Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. The FEBS Journal, 6114-6127. |
| 23486974 | not listed | Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. The Journal of Neuroscience, 5040-5052. |