Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Name: Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
Brand: PhosphoSolutions
SKU: 621-GFAP
Price: 360.0 USD
Availability: InStock

from PhosphoSolutions Datasheet Trial Size

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Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

GFAP

Molecular Weight

50 kDa

Go to Full Product Details

Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.

Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa GFAP protein.

Immunofluorescence of a section of mouse prefrontal cortex labeled with Anti-GFAP(cat. 621-GFAP, red, 1:1000). Image courtesy Andrea Cardenas, Rosalind Franklin Unversity, Medicine and Science.

Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP(cat. 621-GFAP, 1:1000, red), and nuclear staining with DAPI (blue).

Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.

Volume: 100 µL

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Price:

$360

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Product Details

Target

Glial Fibrillary Acidic Protein (GFAP)

Target Description

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

GFAP

Molecular Weight

50 kDa

Antigen

Human recombinant protein

Species Reactivity

Rat

Antibody Registry ID

AB_2492125

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:10,000
WB Brain: 1:10,000
IHC: 1:1000-1:5000
ICC: 1:1000-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~50 kDa GFAP protein. A lower band at ~45 kDa is a proteolytic fragment derived from the GFAP molecule.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

wu:fb34h11 antibody, ALXDRD antibody, cb345 antibody, etID36982.3 antibody, FLJ42474 antibody, FLJ45472 antibody, GFAP antibody, GFAP_HUMAN antibody, gfapl antibody, Glial fibrillary acidic protein antibody, Intermediate filament protein antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody

UniProt Details

UniProt (Human): P14136
UniProt (Immunogen Species): Q28115

NCBI Gene ID

281189

Product Specific References for Applications and Species

Immunohistochemistry: Human | Mouse

Immunohistochemistry: Human
PMID	Dilution	Publication
PMID	Dilution	Publication
34296180	1:250	Levenga, J., et al. 2021. Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity that May Underlie AKT’s Role in Complex Brain Disorders and Neurological Disease. Cerebral Cortex Communications.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
PMID	Dilution	Publication
36443249	1:1000	Milstead, R.A., et al. 2022. TDP-43 Knockdown In Mouse Model Of ALS Leads To Dsrna Deposition, Gliosis, And Neurodegeneration In The Spinal Cord. Cerebral Cortex, .
34296180	1:1000	Levenga, J., et al. 2021. Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity that May Underlie AKT’s Role in Complex Brain Disorders and Neurological Disease. Cerebral Cortex Communications.
29173281	1:1000	Levenga, J., et al. 2017. AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity. eLife, 6, Nov 27. pii: e30640.

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Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction. It is great for use in WB, IHC, ICC.