Name: Anti-GABAA Receptor ß3 Antibody
Brand: PhosphoSolutions
SKU: 863A-GB3C
Price: 415.0 USD
Availability: InStock

Our Anti-GABAA Receptor ß3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor ß3 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GABRB3

Molecular Weight

53 kDa

Go to Full Product Details

Immunofluorescence of mouse retina showing staining of GABAA-R, β3-subunit (cat. : 863A-GB3C, green, 1:300) and calbindin (red). The blue is DAPI staining nuclear DNA. Photo courtesy of Dr. Arlene Hirano, UCLA.

Western blot of rat cortical neurons showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R (1:1000). Image kindly provided by Lidong Liu, University of British Columbia, Vancouver.

$Immunoblots showing GABAA Receptor α2 (cat. 822-GA2CL) and GABAA Receptor ß3 (cat. 863A-GB3C) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.Image from publication CC-BY-4.0. PMID: 36323250$

Western blot of rat brain lysate showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R.

$Immunoblots showing GABAA Receptor α2 (cat. 822-GA2CL) and GABAA Receptor ß3 (cat. 863A-GB3C) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.Image from publication CC-BY-4.0. PMID: 36323250$

Volume: 100 µL

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Price:

$415

Quantity:

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Product Details

Target

GABAA Receptor ß3

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

GABRB3

Molecular Weight

53 kDa

Antigen

Fusion protein from the cytoplasmic loop of the beta 3 subunit of rat GABAA receptor.

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2650497

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:300

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~53 kDa β3-subunit of the GABAA receptor.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ECA5 antibody, GABA alpha receptor beta-2 subunit antibody, GABA(A) receptor subunit beta-3 antibody, GABAA receptor beta 3 subunit antibody, GABAA receptor subunit beta 3 antibody, GABR B3 antibody, Gabrb3 antibody, Gamma aminobutyric acid (GABA) A receptor beta 3 antibody, Gamma aminobutyric acid receptor subunit beta 3 antibody, Gamma-aminobutyric acid receptor subunit beta-3 antibody, GBRB3_HUMAN antibody, MGC9051 antibody

UniProt Details

UniProt (Immunogen Species): P63079

NCBI Gene ID

24922

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
37536384	not listed	Lorenz-Guertin, JM, et al. 2023. Inhibitory and Excitatory Synaptic Neuroadaptations in the Diazepam Tolerant Brain. Neurobiology of Disease, 106248.

Western Blot: Rat
PMID	Dilution	Publication
36323250	not listed	Chan, E.S., et al. 2022. Allosteric potentiation of GABAA Receptor Single-Channel Conductance by Netrin-1 During Neuronal-Excitation-Induced Inhibitory Synaptic Homeostasis. Cell Reports, 111584.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-GABAA Receptor ß3 Antibody

Our Anti-GABAA Receptor ß3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor ß3 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.