Anti-GABAA Receptor, ß3 (Ser408/409) Antibody
Our Anti-GABAA Receptor, ß3 (Ser408/409) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor, ß3 (Ser408/409) and is antigen affinity purified from pooled serum. It is great for use in WB.
![Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).](http://www.phosphosolutions.com/cdn/shop/files/p1130-4089-WB-CNC_c0237409-90f6-40f9-a4f0-683f4a2e6683_{width}x.jpg?v=1692732506)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa GABAA β3 protein phosphorylated at Ser408/409 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
SKU: p1130-4089
Product Details
GABAA Receptor, ß3 (Ser408/409)
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the β3 subunit have been shown to be critical for the functional modulation of β3 containing recombinant receptors (Brandon et al., 2000).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GABRB3
53 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 of the ß3 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish
AB_2492111
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~53 kDa GABAA receptor 3 subunit phosphorylated at Ser408/409. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser408,409
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
ECA5 antibody, GABA alpha receptor beta-2 subunit antibody, GABA(A) receptor subunit beta-3 antibody, GABAA receptor beta 3 subunit antibody, GABAA receptor subunit beta 3 antibody, GABR B3 antibody, Gabrb3 antibody, Gamma aminobutyric acid (GABA) A receptor beta 3 antibody, Gamma aminobutyric acid receptor subunit beta 3 antibody, Gamma-aminobutyric acid receptor subunit beta-3 antibody, GBRB3_HUMAN antibody, MGC9051 antibody
UniProt (Human): P28472
UniProt (Immunogen Species): P63079
UniProt (Immunogen Species): P63079
24922
Product Specific References for Applications and Species
Western Blot: Mouse | ||
PMID | Dilution | Publication |
30077648 | not listed | Brewer,A.L., et al. 2018. Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen. Brain research, 1699, pp.107-116. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
36787750 | 1:500 | Rosenberg, E.C., et al. 2023. Cannabidiol Modulates Excitatory-Inhibitory Ratio to Counter Hippocampal Hyperactivity. Neuron, . |
36233065 | 1:500 | Arenas, Y.M., et al. 2022. Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia. International Journal of Molecular Sciences, . |
25274828 | not listed | Riffault, B., et al. 2014. Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors. The Journal of Neuroscience, 34(40), 13516-13534. |
Product Specific Protocols
- Western Blot Protocol: Download