Our Anti-GABAA Receptor α3 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α3 and is antigen affinity purified. It is great for use in WB, IHC.
Western blot of mouse brain lysates from wild type (WT) and α3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α3-knockout animals.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Antigen Affinity Purified
Synthetic peptide from the N-terminal region of the α3 subunit of rat GABAA receptor.