Name: Anti-GABAA Receptor α3 Antibody
Brand: PhosphoSolutions
SKU: 824-GA3N
Price: 415.0 USD
Availability: InStock

Our Anti-GABAA Receptor α3 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α3 and is antigen affinity purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Zebrafish

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

GABRA3

Molecular Weight

51 kDa

Go to Full Product Details

Immunostaining of rat amygdala showing labeling of GABAA α3 subunit (DAB, 1:100).

Western blot of mouse brain lysates from wild type (WT) and α3-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α3-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α3-knockout animals.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAA Receptor α3 Antibody

Product Details

Target

GABAA Receptor α3

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

GABRA3

Molecular Weight

51 kDa

Antigen

Synthetic peptide from the N-terminal region of the α3 subunit of rat GABAA receptor.

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492102

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~51 kDa α3-subunit of the GABAA receptor. Immunolabeling is absent in α3-subunit knockout animals.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GABA A Receptor alphα3 antibody, GABA(A) receptor subunit alphα3 antibody, GABA(A) receptor subunit alpha-3 antibody, GABR A3 antibody, GABRα3 antibody, Gabra3 antibody, Gamma aminobutyric acid (GABA) A receptor alphα3 antibody, Gamma aminobutyric acid A receptor alphα3 antibody, Gamma aminobutyric acid receptor subunit alphα3 antibody, Gamma-aminobutyric acid receptor subunit alpha-3 antibody, GBRA3_HUMAN antibody, MGC33793 antibody

UniProt Details

UniProt (Human): P34903
UniProt (Immunogen Species): P20236

NCBI Gene ID

24947

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
26446222***	Not listed	Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-GABAA Receptor α3 Antibody

Our Anti-GABAA Receptor α3 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α3 and is antigen affinity purified. It is great for use in WB, IHC.