Anti-GABAA Receptor α2 Antibody

Name: Anti-GABAA Receptor α2 Antibody
Brand: PhosphoSolutions
SKU: 822-GA2CL
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet KO Validated Trial Size Pooled Serum

1 Citation

Our Anti-GABAA Receptor α2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse GABAA Receptor α2 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Rat, Zebrafish

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GABRA2

Molecular Weight

51 kDa

Go to Full Product Details

Western blot of rat cortical neurons showing specific immunolabeling of the ~51 kDa α2-subunit of the GABAA-R (1:1000). Image kindly provided by Lidong Liu, University of British Columbia, Vancouver.

$Immunoblots showing GABAA Receptor α2 (cat. 822-GA2CL) and GABAA Receptor ß3 (cat. 863A-GB3C) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.Image from publication CC-BY-4.0. PMID: 36323250$

Western blot of mouse brain lysates from wild type (WT) and α2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α2-knockout animals.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAA Receptor α2 Antibody

Product Details

Target

GABAA Receptor α2

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channelassociated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABA_A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

GABRA2

Molecular Weight

51 kDa

Antigen

Fusion protein from the cytoplasmic loop of the α2 subunit of rat GABAA receptor.

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Rat, Zebrafish

Antibody Registry ID

AB_2492101

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~51 kDa α2-subunit of the GABAA receptor in Western blots. Labeling is absent in α2-subunit knockout animals.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GABA A receptor subunit alphα2 antibody, GABA antibody, GABA(A) receptor subunit alphα2 antibody, GABA(A) receptor subunit alpha-2 antibody, GABR A2 antibody, GABRα2 antibody, GABRA2 antibody, GABRA2 protein antibody, Gamma aminobutyric acid (GABA) A receptor alphα2 antibody, Gamma aminobutyric acid A receptor alphα2 antibody, Gamma aminobutyric acid receptor alphα2 subunit antibody, Gamma aminobutyric acid receptor subunit alphα2 antibody, Gamma-aminobutyric acid receptor subunit alpha-2 antibody, GBRA2_HUMAN antibody

UniProt Details

UniProt (Human): P47869
UniProt (Immunogen Species): P23576

NCBI Gene ID

61856

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
36323250	not listed	Chan, E.S., et al. 2022. Allosteric potentiation of GABAA Receptor Single-Channel Conductance by Netrin-1 During Neuronal-Excitation-Induced Inhibitory Synaptic Homeostasis. Cell Reports, 111584.

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Anti-GABAA Receptor α2 Antibody

Our Anti-GABAA Receptor α2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse GABAA Receptor α2 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.