Anti-Fractin Antibody

Name: Anti-Fractin Antibody
Brand: PhosphoSolutions
SKU: 592-FRAC
Price: 375.0 USD
Availability: InStock

8 Citations

Our Anti-Fractin primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human and rat Fractin and is neat serum. It is great for use in WB, IHC, ICC.

$Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin).$

$Fractin (cat. 592-FRAC, 1:5000; red) immunostaining of mouse brain signifying the emergence of damaged/dying neurons in entorhinal cortex (EC). Nuclei stained with DAPI (blue). Final image shows fractin+ cells (red) among YFP+ cells (green) at 7 dpi. Image from publication CC-BY-4.0. PMID:36468693$

Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin).

$Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin).$

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 592-FRAC

Volume: 100 µL

Variant

Price:

$375

Quantity:

Product Details

Target

Fractin

Target Description

Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

fragment of Actin

Molecular Weight

32 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of the 32 kDa actin fragment.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492097

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat Serum

Buffer

Neat Serum

Dilution Ranges

WB: 1:1000
IHC: 1:50-1:1000
ICC: 1:100-1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~32 kDa fractin protein. Shows no reactivity toward intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases. In IHC the antibody detects the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Fragment of actin, actin cleavage product

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
9466564	1:100	Yang, F., et al. 1998 . Antibody to caspase-cleaved actin detects apoptosis in differentiated neuroblastoma and plaque-associated neurons and microglia in Alzheimer's disease. The American Journal of Pathology, 152(2), p.379-389.

Immunocytochemistry: Rat
PMID	Dilution	Publication
11516428	1:400	Chen, T.A., et al. 2001. Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Research, 904(1), pp.177-188.
11431120	1:500	Adamec, E., et al. 2001. Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration. Brain Research Protocols, 7(3), pp.193-202.

Immunohistochemistry: Human
PMID	Dilution	Publication
11841033	1:1000	Rossiter, J.P., et al. 2002. Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury. Acta Neuropathologica, 103(1), pp.66-73.
10931367	1:50	Rossiter, J.P., et al. 2000. Caspase‐cleaved actin (fractin) immunolabelling of Hirano bodies. Neuropathology and Applied Neurobiology, 26(4), pp.342-346.
9466564	1:100	Yang, F., et al. 1998 . Antibody to caspase-cleaved actin detects apoptosis in differentiated neuroblastoma and plaque-associated neurons and microglia in Alzheimer's disease. The American Journal of Pathology, 152(2), p.379-389.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36468693	1:5000	Zhao, R., et al. 2022. Activity Disruption Causes Degeneration of Entorhinal Neurons in a Mouse Model of Alzheimer's Circuit Dysfunction. eLife, e83813.

Immunohistochemistry: Rat
PMID	Dilution	Publication
11516428	1:400	Chen, T.A., et al. 2001. Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Research, 904(1), pp.177-188.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Fractin Antibody

SKU: 592-FRAC

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

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