Name: Anti-FMRP (Ser499) Antibody
Brand: PhosphoSolutions
SKU: p1125-499
Price: 415.0 USD
Availability: InStock

Our Anti-FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Chicken, Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate, Sheep, Xenopus, Zebrafish

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

FMR1

Molecular Weight

71 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71 kDa FMRP protein phosphorylated at Ser499 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-FMRP (Ser499) Antibody

Product Details

Target

FMRP (Ser499)

Target Description

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser-499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

FMR1

Molecular Weight

71 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Chicken, Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate, Sheep, Xenopus, Zebrafish

Antibody Registry ID

AB_2492094

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:250-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser499

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FMR 1 antibody, Fmr1 antibody, Fmr1 gene antibody, FMR1_HUMAN antibody, FMRP antibody, Fragile X mental retardation 1 antibody, Fragile X mental retardation 1 protein antibody, Fragile X mental retardation protein 1 antibody, Fragile X mental retardation protein antibody, fragile X mental retardation syndrome-related protein 1 antibody, fragile X mental retardation autosomal homolog 1 antibody, FRAXA antibody, fxr1 antibody, MGC87458 antibody, POF antibody, POF1 antibody, Protein FMR-1 antibody, Protein FMR1 antibody, wu:fb16f11 antibody, wu:fd18c10 antibody, zgc:66226 antibody

UniProt Details

UniProt (Human): Q06787
UniProt (Immunogen Species): Q80WE1

NCBI Gene ID

24948

Product Specific References for Applications and Species

Immunohistochemistry: Chicken | Human | Mouse
Western Blot: Chicken | Human | Mouse | Rat

Immunohistochemistry: Chicken
PMID	Dilution	Publication
26719241	1:1000	Guimarães-Souza, E.M., et al. 2016. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.

Immunohistochemistry: Human
PMID	Dilution	Publication
30630951	1:250-1:1000	Chen, Y., et al. 2019. Aggregation of the nucleic acid–binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation. Journal of Biological Chemistry, 294(10), pp.3696-3706.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35728596	1:200	Monday, H.R., et al. 2022. Presynaptic FMRP and local protein synthesis support structural and functional plasticity of glutamatergic axon terminals. Neuron, .
26719241	1:1000	Guimarães-Souza, E.M., et al. 2016. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.

Western Blot: Chicken
PMID	Dilution	Publication
32449186	not listed	Yu, X., et al. 2020. Dynamics of the Fragile X Mental Retardation Protein Correlates with Cellular and Synaptic Properties in Primary Auditory Neurons following Afferent Deprivation. Journal of Comparative Neurology.
26719241	1:2000	Guimarães-Souza, E.M., et al. 2016. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.

Western Blot: Human
PMID	Dilution	Publication
30630951	1:1000	Chen, Y., et al. 2019. Aggregation of the nucleic acid–binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation. Journal of Biological Chemistry, 294(10), pp.3696-3706.

Western Blot: Mouse
PMID	Dilution	Publication
36067823	1:1000	Ardourel, M., et al. 2022. FMRP-related Retinal Phenotypes: Evidence of Glutamate-Glutamine Metabolic Cycle Impairment. Experimental Eye Research, 109238.
35191199	not listed	Yildirim, Z., et al. 2019. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression. EMBO Molecular Medicine, p.e15344.
31112584	1:1000	DeMarco, B., et al. 2019. FMRP-G-quadruplex mRNA-miR-125a interactions: Implications for miR-125a mediated translation regulation of PSD-95 mRNA. PloS One, 14(5), p.e0217275.
27957526	1:1000	Bartley, C.M., et al. 2016. Mammalian FMRP S499 is phosphorylated by CK2 and promotes secondary phosphorylation of FMRP. eNeuro, 3(6).
26719241	1:2000	Guimarães-Souza, E.M., et al. 2016. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Experimental Eye Research, 146, pp.72-82.
26580204	1:500	Reynolds, C.D., et al. 2015. FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS One, 9(5), p.e96956.
24806451	not listed	Bartley, C.M., et al. 2014. FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS One, 9(5), p.e96956.

Western Blot: Rat
PMID	Dilution	Publication
37494191	1:500	Winden, KD, et al. 2023. Increased Degradation of FMRP Contributes to Neuronal Hyperexcitability in Tuberous Sclerosis Complex. Cell reports, 112838.
23831253	not listed	Bernard, P.B., et al. 2013. Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiology of Disease, 59, pp.1-17.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-FMRP (Ser499) Antibody

Our Anti-FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.