Our Anti-ERK/MAPK rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 kDa ERK/MAPK protein.
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Antigen Affinity Purified from Pooled Serum
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat ERK, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~42-44 kDa ERK/MAPK protein. Immunolabeling is blocked by preadsorption with the peptide used as antigen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.