Name: Anti-ERK/MAPK Antibody
Brand: PhosphoSolutions
SKU: 500-ERK
Price: 415.0 USD
Availability: InStock

Our Anti-ERK/MAPK rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MAPK1/2

Molecular Weight

42/44 kDa

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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 kDa ERK/MAPK protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-ERK/MAPK Antibody

Product Details

Target

ERK/MAPK

Target Description

Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MAPK1/2

Molecular Weight

42/44 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat ERK, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492089

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~42-44 kDa ERK/MAPK protein. Immunolabeling is blocked by preadsorption with the peptide used as antigen.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody

UniProt Details

UniProt (Human): P28482
UniProt (Immunogen Species): P63086

NCBI Gene ID

116590

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
27743527	1:250	Erickson, C.A., et al. 2017. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.

Western Blot: Human
PMID	Dilution	Publication
27743527	1:250	Erickson, C.A., et al. 2017. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-ERK/MAPK Antibody

Our Anti-ERK/MAPK rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.