Name: Anti-EphrinB (Tyr317) Antibody
Brand: PhosphoSolutions
SKU: p1110-317
Price: 415.0 USD
Availability: InStock

Our Anti-EphrinB (Tyr317) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat EphrinB (Tyr317) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Human, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

EPHB2

Molecular Weight

46 kDa

Go to Full Product Details

Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr317 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-EphrinB (Tyr317) Antibody

Product Details

Target

EphrinB (Tyr317)

Target Description

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

EPHB2

Molecular Weight

46 kDa

Antigen

Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Human, Xenopus, Zebrafish

Antibody Registry ID

AB_2492087

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr317. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr317

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody

UniProt Details

UniProt (Human): P29323
UniProt (Immunogen Species): P28693

NCBI Gene ID

396513

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
19995908	not listed	Xing, W., et al. 2010. Ephrin B1 regulates bone marrow stromal cell differentiation and bone formation by influencing TAZ transactivation via complex formation with NHERF1. Molecular and Cellular Biology, 30(3), pp.711-721.

Product Specific Protocols

Western Blot Protocol: Download

Recover password

Create my account

Anti-EphrinB (Tyr317) Antibody

Our Anti-EphrinB (Tyr317) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat EphrinB (Tyr317) and is antigen affinity purified from pooled serum. It is great for use in WB.