Name: Anti-EphrinB (Tyr298) Antibody
Brand: PhosphoSolutions
SKU: p1110-298
Price: 415.0 USD
Availability: InStock

Our Anti-EphrinB (Tyr298) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat EphrinB (Tyr298) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate, Mouse, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

EPHB2

Molecular Weight

46 kDa

Go to Full Product Details

Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr298 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-EphrinB (Tyr298) Antibody

Product Details

Target

EphrinB (Tyr298)

Target Description

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr-298) plays a key role in EphrinB signaling (Kalo, et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

EPHB2

Molecular Weight

46 kDa

Antigen

Synthetic phosphopeptide corresponding to amino acid residues surrounding the Tyr298 of Xenopus EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate, Mouse, Xenopus, Zebrafish

Antibody Registry ID

AB_2492086

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46 kDa EphrinB protein phosphorylated at Tyr298. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr298

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody

UniProt Details

UniProt (Human): P29323
UniProt (Immunogen Species): P28693

NCBI Gene ID

396513

Product Specific Protocols

Western Blot Protocol: Download

General References

Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O., 2004. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein. Biochemical Journal, 377(2), pp.499-507. PMID: 14535844
Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisén, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P., 2003. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron, 39(3), pp.453-465. PMID: 12895420
Flanagan, J.G. and Vanderhaeghen, P., 1998. The ephrins and Eph receptors in neural development. Annual Review of Neuroscience, 21(1), pp.309-345. PMID: 9530499
Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M. and Kisanuki, Y.Y., 2002. Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood, 100(4), pp.1326-1333. PMID: 12149214
Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R., 2002. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Molecular Cell, 9(4), pp.725-737. PMID: 11983165

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Anti-EphrinB (Tyr298) Antibody

Our Anti-EphrinB (Tyr298) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat EphrinB (Tyr298) and is antigen affinity purified from pooled serum. It is great for use in WB.