Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-eEF2K (C-terminus) (lanes 1 & 2), anti-eEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-eEF2K (Ser-398) phospho-specific (lanes 5 & 6).
Anti-eEF2K (Ser-398), Phosphospecific Antibody
Bulk Order Anti-eEF2K (Ser-398), Phosphospecific Antibody
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38δ causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.
Antigen Affinity Purified
Phospho-eEF2K (Ser-398) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-398 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody was cross adsorbed to unphosphorylated eEF2K (Ser-398) peptide before affinity purification using phospho-eEF2K (Ser-398) peptide (without carrier). This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.