Name: Anti-eEF1A2 (Ser358) Antibody
Brand: PhosphoSolutions
SKU: p153-358
Price: 415.0 USD
Availability: InStock

Our Anti-eEF1A2 (Ser358) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eEF1A2 (Ser358) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Expected Reactivity

Chicken, Non-Human Primate, Rat, Sheep, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

EEF1A2

Molecular Weight

50 kDa

Go to Full Product Details

Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~50 kDa eEF1A2 protein phosphorylated at Ser358 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-eEF1A2 (Ser358) Antibody

Product Details

Target

eEF1A2 (Ser358)

Target Description

Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

EEF1A2

Molecular Weight

50 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse

Expected Reactivity

Chicken, Non-Human Primate, Rat, Sheep, Xenopus

Antibody Registry ID

AB_2492084

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser358

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

eEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody

UniProt Details

UniProt (Human): Q05639
UniProt (Immunogen Species): Q05639

NCBI Gene ID

1917

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
23608534	not listed	Gandin, V., et al. 2013. Degradation of newly synthesized polypeptides by ribosome-associated RACK1/c-Jun N-terminal kinase/eukaryotic elongation factor 1A2 complex. Molecular and Cellular Biology, 33(13), pp.2510-2526.

Western Blot: Mouse
PMID	Dilution	Publication
34257105	1:500	Mendoza, M.B., et al. 2021. The elongation factor eEF1A2 controls translation and actin dynamics in dendritic spines. Science Signaling, 14(691), p.eabf5594.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-eEF1A2 (Ser358) Antibody

Our Anti-eEF1A2 (Ser358) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eEF1A2 (Ser358) and is antigen affinity purified from pooled serum. It is great for use in WB.