Name: Anti-Dopamine Transporter, C-Terminus Antibody
Brand: PhosphoSolutions
SKU: 431-DATC
Price: 415.0 USD
Availability: InStock

Our Anti-Dopamine Transporter, C-Terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, non-human primate, and rat Dopamine Transporter, C-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SLC6A3

Molecular Weight

80 kDa

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Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Dopamine Transporter, C-Terminus Antibody

Product Details

Target

Dopamine Transporter, C-Terminus

Target Description

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

SLC6A3

Molecular Weight

80 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region of human dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2492076

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column matrix to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~80 kDa DAT protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DA transporter antibody, DAT 1 antibody, DAT antibody, DAT1 antibody, Dopamine transporter 1 antibody, Dopamine transporter antibody, PKDYS antibody, SC6A3_HUMAN antibody, SLC6A3 antibody, Sodium dependent dopamine transporter antibody, Sodium-dependent dopamine transporter antibody, Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody, Solute carrier family 6 (neurotransmitter transporter) member 3 antibody, Solute carrier family 6 member 3 antibody, Variable number tandem repeat (VNTR) antibody

UniProt Details

UniProt (Human): Q01959
UniProt (Immunogen Species): Q01959

NCBI Gene ID

6531

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Human | Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33345721	1:500	Cuevas, E., et al. 2020. Tauroursodeoxycholic acid (TUDCA) is neuroprotective in a chronic mouse model of Parkinson’s disease. Nutritional Neuroscience, Dec. 21, pp.1-18.

Immunohistochemistry: Rat
PMID	Dilution	Publication
35331847	1:1000	Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp.43-64.
26321151	1:500	Sarkar, S., et al. 2015. Histopathological and electrophysiological indices of rotenone-evoked dopaminergic toxicity: Neuroprotective effects of acetyl-l-carnitine. Neuroscience Letters, 606, pp.53-59.

Western Blot: Human
PMID	Dilution	Publication
31529335	not listed	Rosas-Hernandez, H., et al. 2019. Stretch-Induced Deformation as a Model to Study Dopaminergic Dysfunction in Traumatic Brain Injury. Neurochemical Research, 44(11), pp.2546-2555.
14535941	1.4ug/ml	Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of Neurochemistry, 87(3), pp.574-585.
11282256	not listed	Kish, S.J., et al. 2001. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology, 24(5), p.561.

Western Blot: Rat
PMID	Dilution	Publication
35331847	1:1000	Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp.43-64.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Dopamine Transporter, C-Terminus Antibody

Our Anti-Dopamine Transporter, C-Terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, non-human primate, and rat Dopamine Transporter, C-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.