Name: Anti-DENND3 (Ser554) Antibody
Brand: PhosphoSolutions
SKU: p1030-554
Price: 415.0 USD
Availability: InStock

Our Anti-DENND3 (Ser554) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human DENND3 (Ser554) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate, Mouse, Rat, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

DENND3

Molecular Weight

142 kDa

Go to Full Product Details

Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Ser554 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-DENND3 (Ser554) Antibody

Product Details

Target

DENND3 (Ser554)

Target Description

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (Marat, A.L., et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (Xu and McPherson, 2015; Matsui et al., 2011). ULK-mediated phosphorylation of DENND3 at serines 554 and 572 upregulates its GEF activity toward the small GTPase Rab12 (Xu and McPherson, 2015).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

DENND3

Molecular Weight

142 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser554 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Non-Human Primate, Mouse, Rat, Sheep

Antibody Registry ID

AB_2560942

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Ser554. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser554

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3

UniProt Details

UniProt (Human): A2RUS2
UniProt (Immunogen Species): A2RUS2

NCBI Gene ID

315055

Product Specific Protocols

Western Blot Protocol: Download

General References

Xu, J. and McPherson, P.S., 2015. DENND3: a signaling/trafficking interface in autophagy. Cell Cycle, 14(17), p.2717. PMID: 26177209
Matsui, T., Itoh, T. and Fukuda, M., 2011. Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic, 12(10), pp.1432-1443. PMID: 21718402
Marat, A.L., Dokainish, H. and McPherson, P.S., 2011. DENN domain proteins: regulators of Rab GTPases. Journal of Biological Chemistry, 286(16), pp.13791-13800. PMID: 21330364

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Anti-DENND3 (Ser554) Antibody

Our Anti-DENND3 (Ser554) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human DENND3 (Ser554) and is antigen affinity purified from pooled serum. It is great for use in WB.