Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (CM0191) at 1:500.
Anti-Cyclophilin B Antibody
SKU: CM0191
Bulk Order Anti-Cyclophilin B Antibody
Product Details
Cyclophilin B
Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPI) that are targets of the immunosuppressant drug cyclosporin A. These isomerases have been proposed to aid in protein folding. Cyclophilin B (PPIB) is an ER-localized chaperone protein that associates with type I procollagen, and facilitates procollagen sorting and transport through the secretory compartment. Mutations in the cyclophilin B gene lead to aberrant biosynthesis of type I procollagen, and osteogenesis imperfecta, a disorder characterized by bone fragility. Cyclophilin B has also been implicated as a marker in several cancers, including glioblastomas, neuroblastomas, and gastric cancers. In gastric cancer, Stat3 suppression of miR-520d-5p leads to upregulation of its downstream target cyclophilin B, and enables the growth and survival of these cancer cells.
Protein G Purified
Monoclonal
M019
IgG1
ICC, IP, WB
Mouse
PPIB
20
Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
Unconjugated
Clone M019 detects a 20 kDa* band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A. The antibody can be used for western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
