Anti-Crk II (C-terminal region) Antibody (M332)

Name: Anti-Crk II (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM3321
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M332

Applications

IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

CRK

Molecular Weight

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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk II (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Volume: 100 µL

Variant

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$255

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Product Details

Target

Crk II (C-terminal region)

Target Description

The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M332

Isotype

IgG1

Applications

IP, WB

Host Species

Mouse

Gene Name

CRK

Molecular Weight

Antigen

Clone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IP: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of rat PC12 and human Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

cCrk, c-CRK

UniProt Details

UniProt (Human): P46108
UniProt (Immunogen Species): Q64010

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Anti-Crk II (C-terminal region) Antibody (M332)

Anti-Crk II (C-terminal region) Antibody
SKU: CM3321

Bulk Order Anti-Crk II (C-terminal region) Antibody

Product Details

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Anti-Crk II (C-terminal region) Antibody (M332)

Anti-Crk II (C-terminal region) Antibody SKU: CM3321

Bulk Order Anti-Crk II (C-terminal region) Antibody

Product Details

Anti-Crk II (C-terminal region) Antibody
SKU: CM3321