Our Anti-COXIV (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat COXIV (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Anti-COXIV (Ser58) Antibody
COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser-58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~17 kDa COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
AL024441 antibody, COX 4 antibody, COX IV 1 antibody, COX IV antibody, COX IV-1 antibody, Cox4 antibody, COX41_HUMAN antibody, Cox4a antibody, COX4B antibody, COX4I1 antibody, COX4I2 antibody, COX4L2 antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, Cytochrome C Oxidase subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) antibody, Cytochrome c oxydase subunit 4 antibody, MGC105470 antibody, MGC72016 antibody
UniProt (Immunogen Species): P19783
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