Name: Anti-COXIV (Ser58) Antibody
Brand: PhosphoSolutions
SKU: p1008-58
Price: 415.0 USD
Availability: InStock

Our Anti-COXIV (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat COXIV (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

COX4i1

Molecular Weight

17 kDa

Go to Full Product Details

Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-COXIV (Ser58) Antibody

Product Details

Target

COXIV (Ser58)

Target Description

COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser-58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

COX4i1

Molecular Weight

17 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2492065

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~17 kDa COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser58

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AL024441 antibody, COX 4 antibody, COX IV 1 antibody, COX IV antibody, COX IV-1 antibody, Cox4 antibody, COX41_HUMAN antibody, Cox4a antibody, COX4B antibody, COX4I1 antibody, COX4I2 antibody, COX4L2 antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, Cytochrome C Oxidase subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) antibody, Cytochrome c oxydase subunit 4 antibody, MGC105470 antibody, MGC72016 antibody

UniProt Details

UniProt (Immunogen Species): P19783

NCBI Gene ID

12857

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
27973394	1:500	Neviere, R., et al. 2016. Abnormal mitochondrial cAMP/PKA signaling is involved in sepsis-induced mitochondrial and myocardial dysfunction. International journal of molecular sciences, 17(12), p.2075.

Western Blot: Rat
PMID	Dilution	Publication
21641552	not listed	Acin-Perez, R., et al. 2011. Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation. Cell Metabolism, 13, 712-719.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-COXIV (Ser58) Antibody

Our Anti-COXIV (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat COXIV (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB.