Anti-Connexin-43 (C-terminal region) Antibody (M496)

Name: Anti-Connexin-43 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM4961
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Chicken, Human, Mouse, Rat

Clone

M496

Applications

ICC, WB

Host Species

Mouse

Format

Antigen Affinity Purified

Gene Name

GJA1

Molecular Weight

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Western blot analysis of connexin-43 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-connexin-43 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Volume: 100 µL

Variant

Price:

$255

Quantity:

Bulk Order Anti-Connexin-43 (C-terminal region) Antibody

Product Details

Target

Connexin-43 (C-terminal region)

Target Description

Connexin-43 (Cx43, CXA1, Gap Junction α1) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels that cluster together to form gap junctions. Gap junction communication is critical for cell to cell communication during development and regulation of cell growth. Phosphorylation of connexin-43 is important in regulating both the assembly and the function of gap junctions. PKC phosphorylates Ser-368 in connexin-43 after activation of cells with phorbol esters. This phosphorylation event decreases cell-to-cell communication. In addition, the tyrosine kinase, c-Src, can interact with and phosphorylate Tyr-265 in connexin-43 leading to inhibition of gap junction communication. Thus, connexin-43 phosphorylation may be an important mode for regulating gap junctional communication.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M496

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

GJA1

Molecular Weight

Antigen

Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 43 kDa* protein corresponding to the apparent molecular mass of connexin-43 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Gap junction alpha-1 43 GJA1 CXA1

UniProt Details

UniProt (Human): P17302
UniProt (Immunogen Species): P17302

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Anti-Connexin-43 (C-terminal region) Antibody (M496)

Anti-Connexin-43 (C-terminal region) Antibody
SKU: CM4961

Bulk Order Anti-Connexin-43 (C-terminal region) Antibody

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Anti-Connexin-43 (C-terminal region) Antibody (M496)

Anti-Connexin-43 (C-terminal region) Antibody SKU: CM4961

Bulk Order Anti-Connexin-43 (C-terminal region) Antibody

Product Details

Anti-Connexin-43 (C-terminal region) Antibody
SKU: CM4961