Anti-Connexin 35/36 (Ser276) Antibody

Name: Anti-Connexin 35/36 (Ser276) Antibody
Brand: PhosphoSolutions
SKU: pCx35-276
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

Our Anti-Connexin 35/36 (Ser276) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects fish, mouse, and rabbit Connexin 35/36 (Ser276) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Fish, Mouse, Rabbit

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

Cx35

Molecular Weight

35 kDa

Go to Full Product Details

Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.

Volume: 100 uL

Variant

Price:

$415

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Bulk Order Anti-Connexin 35/36 (Ser276) Antibody

Product Details

Target

Connexin 35/36 (Ser276)

Target Description

Connexin 35/36 is the most abundant gap junction protein in the central nervous system. Connexin gap junction coupling in the retina has been shown to be regulated by light exposure and neurotransmitter signaling, but more recently has been observed to also depend on direct phosphorylation of connexin (Ouyang et al., 2005, Patel et al., 2006). PKA phosphorylation of Ser110 in the intracellular loop and Ser276 in the C-terminal domain can cause uncoupling of connexin 35 gap junctions (Ouyang et al., 2005). The largest uncoupling effect is seen when both of these sites are phosphorylated, with a much smaller degree of uncoupling observed when only one is phosphorylated (Kothmann et al., 2007). C-terminal domain phosphorylation of connexin 35 at Ser276 is observed in teleost, amphibian, and avian species, with connexin 36 phosphorylation at Ser293 being the mammalian equivalent.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

Cx35

Molecular Weight

35 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues of perch connexin 35/36 surrounding Ser276, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Fish, Mouse, Rabbit

Antibody Registry ID

AB_2857923

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~35 kDa connexin-35/36 protein phosphorylated at Ser276.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser276

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Connexin 36 / GJA9 antibody, Connexin 36 antibody, Connexin-36 antibody, Cx36 antibody, CXD2_HUMAN antibody, Gap junction alpha-9 protein antibody, Gap junction delta-2 protein antibody, Gap junction protein alpha 9 antibody, gap junction protein, alpha 9, 36kDa antibody, JA9 antibody, GJD2 antibody, MGC138315 antibody, MGC138319 antibody, Connexin 35 antibody, Connexin-35 antibody, Cx35 antibody, Cx35.4 antibody

UniProt Details

UniProt (Immunogen Species): O93328

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Connexin 35/36 (Ser276) Antibody

Our Anti-Connexin 35/36 (Ser276) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects fish, mouse, and rabbit Connexin 35/36 (Ser276) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.