Anti-Cdk5 Antibody (DC17+DC34)

Name: Anti-Cdk5 Antibody
Brand: PhosphoSolutions
SKU: CM2361
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

DC17+DC34

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

CDK5

Molecular Weight

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Western blot analysis of human HeLa cells. The blot was probed with anti-Cdk5 (CM2361) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000.(lane 3).

Volume: 100 µL

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$255

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Product Details

Target

Cdk5

Target Description

Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.

Format

Protein A Purified

Clonality

Monoclonal

Clone

DC17+DC34

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

CDK5

Molecular Weight

Antigen

Clones DC17+DC34 were generated from a recombinant full-length human Cdk5 sequence.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 31 kDa* band corresponding to Cdk5 on SDS-PAGE immunoblots of human SYF and HeLa cells, and in mouse brain tissue. The antibody does not detect other Cdk family members, such as Cdk1 and Cdk2.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PSSALRE, TPKII

UniProt Details

UniProt (Human): Q00535
UniProt (Immunogen Species): Q00535

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Anti-Cdk5 Antibody (DC17+DC34)

Anti-Cdk5 Antibody
SKU: CM2361

Bulk Order Anti-Cdk5 Antibody

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