Western blot of UV treated HeLa cell lysate showing specific immunolabeling of the ~98 kDa CD71 protein.
CD71, also known as the transferrin receptor (TfR1), is a transmembrane glycoprotein whose primary function is to mediate cellular uptake of iron from a plasma glycoprotein, transferrin. CD71 is a widely used marker for cell activation or proliferation of lymphocytes as transferrin receptor expression is undetectable in resting T and B lymphocytes, but increases significantly upon lymphocyte antigen activation (Galbraith, R.M., et al 1981). Rapidly proliferating cells and energy-requiring cells, like cancer cells, osteoclasts (Roodman, G.D., 2009), and activated lymphocytes (Wieland, E., et al, 2016) express high levels of CD71 due to increased iron demand. TfR1 is seen as an efficient drug delivery system for cancer patients as it can mediate extracellular transferrin conjugated molecules for treatment (Luria-Perez, R., et al, 2016).
Protein G purified
Preparation of KG1 cells
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Protein G purified culture supernatant.
Specific for endogenous levels of the ~98 kDa CD71 protein.
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After date of receipt, stable for at least 1 year at -20°C.