Anti-CD46 (Extracellular region) Antibody (M037)

Name: Anti-CD46 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: CM0371
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M037

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

CD46

Molecular Weight

45-60

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Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.

Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (CM0371). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD46 (CM0371) for ELISA capture of human recombinant CD46 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary
antibody HRP conjugate.

Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.

Volume: 100 µL

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Price:

$340

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Product Details

Target

CD46 (Extracellular region)

Target Description

CD46 is a complement regulatory protein that is also called membrane cofactor protein. This protein is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host cells from complement-dependent cytotoxicity. CD46 can also function as a receptor for selected bacteria and viruses, and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization. CD46 is overexpressed in medulloblastoma tumors, and CD46 expression has been linked with poor prognosis in breast cancer. The upregulation of CD46 may protect cancer cells from complement-dependent cytotoxicity to facilitate cancer cell immune evasion.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M037

Isotype

IgG2b

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

CD46

Molecular Weight

45-60

Antigen

Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M037 mouse monoclonal antibody detects a 45-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, MCP, MIC10

UniProt Details

UniProt (Human): P15529
UniProt (Immunogen Species): P15529

Product Specific Protocols

Western Blot Protocol: Download

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Anti-CD46 (Extracellular region) Antibody (M037)

Anti-CD46 (Extracellular region) Antibody
SKU: CM0371

Bulk Order Anti-CD46 (Extracellular region) Antibody

Product Details

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Anti-CD46 (Extracellular region) Antibody (M037)

Anti-CD46 (Extracellular region) Antibody SKU: CM0371

Bulk Order Anti-CD46 (Extracellular region) Antibody

Product Details

Product Specific Protocols

Anti-CD46 (Extracellular region) Antibody
SKU: CM0371