Our Anti-CaSR rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CaSR and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of lysed HFF cells that were serum starved for 24 hours and then refed for 30 minutes showing specific immunolabeling of the ~130-260 kDa CaSR protein.
Calcium sensing receptor, CaSR, is a unique G protein-coupled receptor activated by extracellular Ca2+, Mg2+, Sr2+, Mn2+, Ba2+, Ni2+, Al2+, Gd3+, amino acids, polyamines, pH and ionic strength (Quinn, S.J. et al., 1998). CaSR is a cell surface dimer located in the caveolin-rich plasma membrane domains (Kifor, O., et al., 1998). CaSR tightly regulates extracellular calcium levels to avoid hyper/hypo-calcemia through complex interactions with PTH, calcitonin, and vitamin D by balancing their effects on the parathyroid glands, bone, kidney, and intestine (Díaz-Soto, G. et al., 2016). Additionally, CaSR has been identified in the nervous system and recent studies suggest CaSR as a direct target of reducing Aβ42 secretion that may contribute to Alzheimer’s Disease (Armato, U., et al., 2013). Recent studies have shown CaSR negatively impacts the formation and progression of neuroblastomas by calcium exposure leading NB cells overexpressing CaSR to undergo apoptosis by means of activating ERK1/2 (Casalà, C., et al., 2013).
Antigen Affinity Purified from Pooled Serum
Synthetic peptide from the N-terminus region of rat calcium sensing receptor, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification on a column made with the N-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~130-260 kDa CaSR protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.