Anti-C-Raf (S471) [B-Raf (S579)/A-Raf (S432)], Phosphospecific Antibody

Name: Anti-C-Raf (S471) [B-Raf (S579)/A-Raf (S432)], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: RP2901
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

RAF1

Molecular Weight

Go to Full Product Details

Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-C-Raf (N-terminal region) (lanes 1 & 2) or anti-C-Raf (Ser-471) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-C-Raf (S471) [B-Raf (S579)/A-Raf (S432)], Phosphospecific Antibody

Product Details

Target

C-Raf (S471)[B-Raf (S579)/A-Raf (S432)]

Target Description

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and Ser-471. The latter site is phosphorylated after EGF stimulation and may be important for MEK interaction in both C-Raf and A-Raf. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

RAF1

Molecular Weight

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-C-Raf (Ser-338) peptide before affinity purification using phospho-C-Raf (Ser-471) peptide. The purified antibody detects a band at 74 kDa* corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-471 [S578/S433]

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Raf1, CRaf

UniProt Details

UniProt (Human): P04049
UniProt (Immunogen Species): P04049

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Anti-C-Raf (S471) [B-Raf (S579)/A-Raf (S432)], Phosphospecific Antibody