Anti-C-Raf (N-terminal region) Antibody (M208)

Name: Anti-C-Raf (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: RM2081
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M208

Applications

Host Species

Mouse

Format

Protein G Purified

Gene Name

RAF1

Molecular Weight

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Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-C-Raf (N-terminal) antibody at 1:500.

Volume: 100 µL

Variant

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$255

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Bulk Order Anti-C-Raf (N-terminal region) Antibody

Product Details

Target

C-Raf (N-terminal region)

Target Description

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation

Format

Protein G Purified

Clonality

Monoclonal

Clone

M208

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

RAF1

Molecular Weight

Antigen

Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Raf1, CRaf

UniProt Details

UniProt (Human): P04049
UniProt (Immunogen Species): P04049

Product Specific Protocols

Western Blot Protocol: Download

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Anti-C-Raf (N-terminal region) Antibody (M208)

Anti-C-Raf (N-terminal region) Antibody
SKU: RM2081

Bulk Order Anti-C-Raf (N-terminal region) Antibody

Product Details

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Anti-C-Raf (N-terminal region) Antibody (M208)

Anti-C-Raf (N-terminal region) Antibody SKU: RM2081

Bulk Order Anti-C-Raf (N-terminal region) Antibody

Product Details

Product Specific Protocols

Anti-C-Raf (N-terminal region) Antibody
SKU: RM2081