Name: Anti-Beclin1 (Ser295) Antibody
Brand: PhosphoSolutions
SKU: p117-295
Price: 415.0 USD
Availability: InStock

Our Anti-Beclin1 (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Beclin1 (Ser295) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

BECN1

Molecular Weight

60 kDa

Go to Full Product Details

Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser295. This immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A: Mut) expressed in HeLa cell lysate.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Beclin1 (Ser295) Antibody

Product Details

Target

Beclin1 (Ser295)

Target Description

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser-295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

BECN1

Molecular Weight

60 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Antibody Registry ID

AB_2492048

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser295

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PG6 antibody, ATG 6 antibody, ATG6 antibody, ATG6 autophagy related 6 homolog antibody, Bcl-2-interacting protein beclin antibody, Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody, Beclin 1 autophagy related antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody

UniProt Details

UniProt (Human): Q14457
UniProt (Immunogen Species): Q14457

NCBI Gene ID

8678

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
27696537	1:250	Weh, K.M., et al. 2016. Expression, modulation, and clinical correlates of the autophagy protein Beclin‐1 in esophageal adenocarcinoma. Molecular carcinogenesis, 55(11), pp.1876-1885.
23112296	not listed	Wang, R.C., et al. 2012. Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

Western Blot: Mouse
PMID	Dilution	Publication
29669822	not listed	Song, X., et al. 2018. Crosstalk Between Apoptosis and Autophagy Is Regulated by the Arginylated BiP/Beclin-1/p62 Complex. Molecular Cancer Research. Jul;16(7):1077-1091.

Western Blot: Rat
PMID	Dilution	Publication
23112296	not listed	Wang, R.C., et al. 2012. Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Beclin1 (Ser295) Antibody

Our Anti-Beclin1 (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Beclin1 (Ser295) and is antigen affinity purified from pooled serum. It is great for use in WB.