Anti-B7-H3/CD276 (Extracellular region) Antibody (M045)

Name: Anti-B7-H3/CD276 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: BM0451
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M045

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

CD276

Molecular Weight

100-120

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Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.

Immunocytochemical labeling of B7-H3 in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse monoclonal anti-B7-H3/CD276 (BM0451). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-B7-H3 (BM0451) for ELISA capture of human recombinant B7-H3 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Western blot of human LNCaP prostate adenocarcinoma (lane 1), MCF-7 breast ductal carcinoma (lane 2), NCI-H28 pleural mesothelioma (lane 3), A431 epidermoid carcinoma (lane 4), MDAMB-231 breast carcinoma (lane 5) and Jurkat T-cell line (lane 6). The blot was probed with mouse monoclonal anti-B7-H3/CD276 (BM0451) at 1:500.

Volume: 100 µL

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Price:

$340

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Bulk Order Anti-B7-H3/CD276 (Extracellular region) Antibody

Product Details

Target

B7-H3/CD276 (Extracellular region) Antibody

Target Description

B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a membrane protein with an extracellular region that includes two Ig-like V-type domains and two IgG-like C2-type domains, and a short intracellular domain. B7-H3 is a regulatory molecule for immune reactions, such as T cell proliferation and IFN-γ production. In colon cancers, B7-H3 inhibits T-cell cytotoxicity, and blocking B7-H3 function enhances T-cell cytotoxicity toward cancer cells. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. In cancer, B7-H3 is expressed in various tumor types including prostate, breast, colon, lung, and gastric cancers. The B7-H3 expression level correlates with tumor growth, invasion, metastasis, malignant stage, and recurrence rate. The inhibition or blockade of B7-H3 function could be an important immunotherapeutic approach for several types of cancer

Format

Protein G Purified

Clonality

Monoclonal

Clone

M045

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

CD276

Molecular Weight

100-120

Antigen

Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CD276, B7-H3, B7H3, B7RP-2, B7 homolog 3

UniProt Details

UniProt (Human): Q5ZPR3
UniProt (Immunogen Species): Q5ZPR3

Product Specific Protocols

Western Blot Protocol: Download

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Anti-B7-H3/CD276 (Extracellular region) Antibody (M045)

Anti-B7-H3/CD276 (Extracellular region) Antibody
SKU: BM0451

Bulk Order Anti-B7-H3/CD276 (Extracellular region) Antibody

Product Details

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Anti-B7-H3/CD276 (Extracellular region) Antibody (M045)

Anti-B7-H3/CD276 (Extracellular region) Antibody SKU: BM0451

Bulk Order Anti-B7-H3/CD276 (Extracellular region) Antibody

Product Details

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Anti-B7-H3/CD276 (Extracellular region) Antibody
SKU: BM0451