Name: Anti-ATF2 (Ser490,498) Antibody
Brand: PhosphoSolutions
SKU: p115-4908
Price: 415.0 USD
Availability: InStock

Our Anti-ATF2 (Ser490,498) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Ser490,498) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse

Expected Reactivity

Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

ATF2

Molecular Weight

74 kDa

Go to Full Product Details

Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-ATF2 (Ser490,498) Antibody

Product Details

Target

ATF2 (Ser490,498)

Target Description

The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003; Morton et al., 2004). Stress induced ATFdependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000); Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

ATF2

Molecular Weight

74 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 of human ATF2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse

Expected Reactivity

Rat

Antibody Registry ID

AB_2492044

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~74 kDa ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser490,498

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Activating transcription factor 2 antibody, Activating transcription factor 2 splice variant ATF2 var2 antibody, ATF 2 antibody, Atf-2 antibody, Atf2 antibody, ATF2 protein antibody, ATF2_HUMAN antibody, cAMP Response Element Binding Protein 2 antibody, cAMP response element binding protein CRE BP1 antibody, cAMP response element-binding protein CRE-BP1 antibody, cAMP responsive element binding protein 2, formerly antibody, cAMP-dependent transcription factor ATF-2 antibody, cAMP-responsive element-binding protein 2 antibody, CRE BP1 antibody, CRE-BP antibody, CREB 2 antibody, CREB-2 antibody, CREB2 antibody, CREBP1 antibody, Cyclic AMP dependent transcription factor ATF 2 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, D130078H02Rik antibody, D18875 antibody, HB 16 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, MGC105211 antibody, MGC105222 antibody, MGC111558 antibody, MGC142504 antibody, mXBP antibody, MXBP protein antibody, Tg(Gzma-Klra1)7Wum antibody, TREB 7 antibody, TREB7 antibody

UniProt Details

UniProt (Human): P15336
UniProt (Immunogen Species): P15336

NCBI Gene ID

1386

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
23325849	not listed	Muñoz, D.P., et al. 2013. An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells. Nucleic acids research 41.5 (2013): 2894-2906.
15916964	1:500	Bhoumik, A., et al. 2005. ATM-dependent phosphorylation of ATF2 is required for DNA damage response. Mol Cell, May 27; 18(5):577-87.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
15916964	1:500	Bhoumik, A., et al. 2005. ATM-dependent phosphorylation of ATF2 is required for DNA damage response. Mol Cell, May 27; 18(5):577-87.

Western Blot: Human
PMID	Dilution	Publication
15916964	not listed	Bhoumik, A., et al. 2005. ATM-dependent phosphorylation of ATF2 is required for DNA damage response. Mol Cell, May 27; 18(5):577-87.

Western Blot: Mouse
PMID	Dilution	Publication
15916964	not listed	Bhoumik, A., et al. 2005. ATM-dependent phosphorylation of ATF2 is required for DNA damage response. Mol Cell, May 27; 18(5):577-87.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-ATF2 (Ser490,498) Antibody

Our Anti-ATF2 (Ser490,498) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse ATF2 (Ser490,498) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.