Our Arpc1b/p41-Arc (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Arpc1b/p41-Arc (C-terminal region) and is antigen affinity purified. It is great for use in WB, IP, ICC.
Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21
Antigen Affinity Purified
Polyclonal
IgG
ICC, IP, WB
Rabbit
ARPC1B
41
Arpc1b/p41-Arc synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arpc1b. This peptide sequence is highly conserved in rat, mouse, and chicken Arpc1b, and has some homology to the conserved region in isoform Arpc1a.
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:200
IP: 1:100
Unconjugated
The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and Jurkat cells, rat A7r5 and PC12 cells, and mouse C2C12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Merz, KE, et al. 2022. Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis. Frontiers in endocrinology, 821849.
Zhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.
Tunduguru, R, et al. 2017. The actin-related p41ARC subunit contributes to p21-activated kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells. The Journal of biological chemistry, 19034-19043.
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