Anti-Argonaute 2 (Tyr-393), Phosphospecific Antibody

Name: Anti-Argonaute 2 (Tyr-393), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP5311
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

3 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

AGO2

Molecular Weight

Go to Full Product Details

Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

Argonaute 2 (Tyr-393)

Target Description

Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

AGO2

Molecular Weight

Antigen

Phospho-Ago2 (Tyr-393) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 393 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. The site is also conserved in Ago1 and Ago4, but not in Ago3.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody was cross-absorbed to unphospho-Ago2 (Tyr-393), before affinity purification using phospho-Ago2 (Tyr-393) peptide. The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells that were treated with EGF. This reactivity is not observed after lambda phosphatase dephosphorylation.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-393

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer

UniProt Details

UniProt (Human): Q9UKV8
UniProt (Immunogen Species): Q9UKV8

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Frog | Human

Immunocytochemistry: Human
PMID	Dilution	Publication
28877994	not listed	Kourtidis, A, et al. 2017. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. The Journal of cell biology, 3073-3085.

Western Blot: Frog
PMID	Dilution	Publication
29681008	1:1000	Hadj-Moussa, H, et al. 2018. Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains. Cellular and molecular life sciences : CMLS, 3635-3647.

Western Blot: Human
PMID	Dilution	Publication
28877994	not listed	Kourtidis, A, et al. 2017. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. The Journal of cell biology, 3073-3085.

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Anti-Argonaute 2 (Tyr-393), Phosphospecific Antibody