Anti-Aquaporin 2 (Ser269) Antibody

Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.



SKU: p112-269

Volume: 100 µL
Price:
Sale price$430

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
33436646 1:200 Flahaunt, A., et al. 2021. A metabolically stable apelin-17 analog decreases AVP-induced antidiuresis and improves hyponatremia. Nature Communications, 12(1), pp.1-14.
Immunocytochemistry: Rat
PMID Dilution Publication
28668390 1:500 Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.
Immunohistochemistry: Mouse
PMID Dilution Publication
18606813 1:2000 Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
Immunohistochemistry: Rat
PMID Dilution Publication
26147297 not listed Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
23559583 1:600 Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.
Immunoprecipitation: Mouse
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
Immunoprecipitation: Rat
PMID Dilution Publication
27889609 3 uL Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
Western Blot: Human
PMID Dilution Publication
32529500 not listed Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
32486031 not listed Ranieri, M., et al. 2020. The Vasopressin Receptor 2 Mutant R137L Linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) Signals through an Alternative Pathway that Increases AQP2 Membrane Targeting Independently of S256 Phosphorylation. Cells, 9(6), p.1354.
Western Blot: Mouse
PMID Dilution Publication
369959261:1000Zuchowski, Y., et al. 2023. The Insulin Regulated Aminopeptidase (IRAP) is Required for Water Excretion in Response to Acute Hypotonic Stress. American Journal of Physiology. Renal Physiology, F521-F531.
363268351:1000Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .
32529500 not listed Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
29046300 1:3000 Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609 1:500-1:1000 Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27707708 1:1000 Gao, Y., et al. 2016. Collecting duct-specific knockout of nitric oxide synthase 3 impairs water excretion in a sex-dependent manner. American Journal of Physiology-Renal Physiology, pp.ajprenal-00494.
18606813 1:2000 Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
Western Blot: Rat
PMID Dilution Publication
370475091:1000Wang, Y., et al. 2023. Phosphatases Decrease Water and Urea Permeability in Rat Inner Medullary Collecting Ducts. International Journal of Molecular Sciences, .
31994353 1:1000 Masuda, T., et al. 2020. Osmotic diuresis by SGLT2 inhibition stimulates vasopressin‐induced water reabsorption to maintain body fluid volume. Physiological Reports, 8(2), p.e14360.
29046300 1:3000 Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390 1:500 Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609 1:500-1:1000 Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994 not listed Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292 not listed Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26574044 1:5000 Mamenko, M., et al. 2016. Defective store-operated calcium entry causes partial nephrogenic diabetes insipidus. Journal of the American Society of Nephrology, 27(7), pp.2035-2048.
26147297 not listed Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
25651562 1:1000 Choi,H. J., et al. 2015. Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48.
24259510 1:1000 Hoffert, J.D., et al. 2014. Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421.
23559583 1:600 Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.

Product Specific Protocols

  • Aquaporin ser269 IHF Protocol: Download

Related Products

Recently Viewed