Name: Anti-Aquaporin 2 (Ser269) Antibody
Brand: PhosphoSolutions
SKU: p112-269
Price: 415.0 USD
Availability: InStock

Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

AQP2

Molecular Weight

29/37 kDa

Go to Full Product Details

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.

Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Aquaporin 2 (Ser269) Antibody

Product Details

Target

Aquaporin 2 (Ser269)

Target Description

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Gene Name

AQP2

Molecular Weight

29/37 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat aquaporin 2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine

Antibody Registry ID

AB_2492043

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:600-1:5000
ICC: 1:200-1:500
IP: 3 ul

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser269

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

UniProt Details

UniProt (Immunogen Species): P34080

NCBI Gene ID

25386

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse | Rat
Immunoprecipitation: Mouse | Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
33436646	1:200	Flahaunt, A., et al. 2021. A metabolically stable apelin-17 analog decreases AVP-induced antidiuresis and improves hyponatremia. Nature Communications, 12(1), pp.1-14.

Immunocytochemistry: Rat
PMID	Dilution	Publication
28668390	1:500	Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.

Immunohistochemistry: Rat
PMID	Dilution	Publication
26147297	not listed	Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
23559583	1:600	Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
27889609	3 uL	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.

Immunoprecipitation: Rat
PMID	Dilution	Publication
27889609	3 uL	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.

Western Blot: Human
PMID	Dilution	Publication
32529500	not listed	Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
32486031	not listed	Ranieri, M., et al. 2020. The Vasopressin Receptor 2 Mutant R137L Linked to the Nephrogenic Syndrome of Inappropriate Antidiuresis (NSIAD) Signals through an Alternative Pathway that Increases AQP2 Membrane Targeting Independently of S256 Phosphorylation. Cells, 9(6), p.1354.

Western Blot: Mouse
PMID	Dilution	Publication
36326835	1:1000	Arroyo, J.P., et al. 2022. Kidney Collecting Duct Cells Make Vasopressin In Response To NaCl Induced Hypertonicity. JCI Insight, .
32529500	not listed	Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
29046300	1:3000	Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609	1:500-1:1000	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27707708	1:1000	Gao, Y., et al. 2016. Collecting duct-specific knockout of nitric oxide synthase 3 impairs water excretion in a sex-dependent manner. American Journal of Physiology-Renal Physiology, pp.ajprenal-00494.
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.

Western Blot: Rat
PMID	Dilution	Publication
31994353	1:1000	Masuda, T., et al. 2020. Osmotic diuresis by SGLT2 inhibition stimulates vasopressin‐induced water reabsorption to maintain body fluid volume. Physiological Reports, 8(2), p.e14360.
29046300	1:3000	Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390	1:500	Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609	1:500-1:1000	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994	not listed	Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292	not listed	Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26574044	1:5000	Mamenko, M., et al. 2016. Defective store-operated calcium entry causes partial nephrogenic diabetes insipidus. Journal of the American Society of Nephrology, 27(7), pp.2035-2048.
26147297	not listed	Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
25651562	1:1000	Choi,H. J., et al. 2015. Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48.
24259510	1:1000	Hoffert, J.D., et al. 2014. Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421.
23559583	1:600	Bogum, J., et al. 2013. Small-molecule screening identifies modulators of aquaporin-2 trafficking. Journal of the American Society of Nephrology, 24(5), 744-758.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Aquaporin 2 (Ser269) Antibody

Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.