Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Drosophila
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
AKT1
68 kDa

Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Product Specific References for Applications and Species
- Western Blot: Drosophila | Mosquito
Western Blot: Drosophila | ||
PMID | Dilution | Publication |
33370287 | 1:500 | Towler, B.P., et al. 2020. Dis3L2 regulates cell proliferation and tissue growth though a conserved mechanism. PLoS Genetics, 16(12), p.e1009297. |
32291952 | 1:1000 | Woodling, N.S., et al. 2020. The neuronal receptor tyrosine kinase Alk is a target for longevity. Aging Cell, p.e13137. |
31061470 | 1:500 | Xu, Y., et al. 2019. Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific Reports, 9(1), p.6989. |
29892262 | 1:1000 | Post, S., et al. 2018. Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28; 9:245. |
29491510 | not listed | Lin, F., et al. 2017. Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212. |
25043021 | 1:200 | Schleich, S., et al. 2014. DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth. Nature. Aug 14; 512(7513):208-12. |
Western Blot: Mosquito | ||
PMID | Dilution | Publication |
35129195 | 1:1000 | Martinson, E., et al. 2022. Insulin-like peptide 3 stimulates hemocytes to proliferate in anautogenous and facultatively autogenous mosquitoes. The Journal of Experimental Biology, 225(5). |
Product Specific Protocols
- Western Blot Protocol: Download