Name: Anti-AKT (Thr342) Antibody
Brand: PhosphoSolutions
SKU: p104-342
Price: 415.0 USD
Availability: InStock

Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Drosophila

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

AKT1

Molecular Weight

68 kDa

Go to Full Product Details

Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-AKT (Thr342) Antibody

Product Details

Target

AKT (Thr342)

Target Description

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

AKT1

Molecular Weight

68 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH)

Species Reactivity

Drosophila

Antibody Registry ID

AB_2492036

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~68 kDa AKT protein phosphorylated at Thr342. Immunolabeling is completely eliminated with λ-phosphatase treatment. It has been reported that this antibody may also recognize some level of phosphorylated S6K as there is 67% homology with the sequence used as antigen.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr342

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AKT1 antibody, AKT1_HUMAN antibody, AKT2 antibody, AKT3 antibody, PKB alpha antibody, PKB antibody, PKB beta antibody, PKBalpha antibody, PRKBA antibody, PRKBB antibody, PRKBG antibody, Protein kinase B antibody, Protein kinase B beta antibody, Protein kinase B gamma antibody, Proto-oncogene c-Akt antibody, RAC alpha antibody, RAC antibody, RAC gamma antibody, RAC PK alpha antibody, RAC PK beta antibody, RAC PK gamma antibody, RAC-alpha serine/threonine-protein kinase antibody, RAC-PK-alpha antibody, STK2 antibody

UniProt Details

UniProt (Immunogen Species): Q8INB9

NCBI Gene ID

41957

Product Specific References for Applications and Species

Western Blot: Drosophila | Mosquito

Western Blot: Drosophila
PMID	Dilution	Publication
33370287	1:500	Towler, B.P., et al. 2020. Dis3L2 regulates cell proliferation and tissue growth though a conserved mechanism. PLoS Genetics, 16(12), p.e1009297.
32291952	1:1000	Woodling, N.S., et al. 2020. The neuronal receptor tyrosine kinase Alk is a target for longevity. Aging Cell, p.e13137.
31061470	1:500	Xu, Y., et al. 2019. Chronic dysfunction of Stromal interaction molecule by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila. Scientific Reports, 9(1), p.6989.
29892262	1:1000	Post, S., et al. 2018. Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Frontiers in Endocrinology, May 28; 9:245.
29491510	not listed	Lin, F., et al. 2017. Total solid-phase synthesis of biologically active drosophila insulin-like peptide 2 (DILP2). Australian Journal of Chemistry, 70(2), pp.208-212.
25043021	1:200	Schleich, S., et al. 2014. DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth. Nature. Aug 14; 512(7513):208-12.

Western Blot: Mosquito
PMID	Dilution	Publication
35129195	1:1000	Martinson, E., et al. 2022. Insulin-like peptide 3 stimulates hemocytes to proliferate in anautogenous and facultatively autogenous mosquitoes. The Journal of Experimental Biology, 225(5).

Product Specific Protocols

Western Blot Protocol: Download

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Anti-AKT (Thr342) Antibody

Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.