Anti-Akt (Thr-34), Phosphospecific Antibody

Name: Anti-Akt (Thr-34), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP1001
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Rabbit, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

AKT1

Molecular Weight

Go to Full Product Details

Western blot analysis of A431 cells, serum starved overnight (20 µg/lanes 1 & 3) and calyculin A (100 nM) treated for 30 minutes (20 µg/lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-Akt (Thr-34), Phosphospecific Antibody

Product Details

Target

Akt (Thr-34)

Target Description

Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

AKT1

Molecular Weight

Antigen

Phospho-Akt (Thr-34) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt.

Species Reactivity

Human, Rabbit, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of phoshorylated Akt on SDS-PAGE immunoblots of A431 + calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-34

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PKBalpha, PKB, AKT

UniProt Details

UniProt (Human): P31749
UniProt (Immunogen Species): P31749

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Akt (Thr-34), Phosphospecific Antibody