Name: Anti-Adenylate Cyclase III Antibody
Brand: PhosphoSolutions
SKU: 85-AC3
Price: 415.0 USD
Availability: InStock

Our Anti-Adenylate Cyclase III primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Adenylate Cyclase III and is antigen affinity purified. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

ADCY3

Molecular Weight

160, 200 kDa

Go to Full Product Details

Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (cat. 85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (cat. 1205-MeCP2, 1:1000,green), and Hoechst staining of nuclear DNA.

Immunostaining of cultured rat neurons and glia showing strong staining of neuronal cilia using our anti-adenylate cyclase III antibody (cat. 85-AC3, 1:500, red) and axonal and dendritic staining of alpha II spectrin (cat. 98-A2SM,1:500, green) revealing the submembranous cytoskeleton and DNA (blue).

Western blot of rat cortical lysate showing specific immunolabeling of the ~160 kDa adenylate cyclase III protein.

Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (cat. 85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (cat. 1205-MeCP2, 1:1000,green), and Hoechst staining of nuclear DNA.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Adenylate Cyclase III Antibody

Product Details

Target

Adenylate Cyclase III

Target Description

Adenylate cyclase is the enzyme which produces the "second messenger" signaling molecule, cAMP from ATP. Type III adenylate cyclase is localized to the membranes surrounding neuronal cilia. Much is currently unknown about the function of primary cilia in vertebrates, however, recent work has begun to explore their role in neuronal signaling and neurogenesis (Fuchs and Schwark, 2004; Louvi and Grove 2011).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

ADCY3

Molecular Weight

160, 200 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat adenylate cyclase III, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492029

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Affinity purified rabbit serum.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:2000
WB Brain: 1:2000
IHC: 1:10,000
ICC: 1:500-1:10,000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~160 kDa adenylate cyclase III protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AC-III antibody, AC3 antibody, ADCY3 antibody, ADCY3_HUMAN antibody, adenylate cyclase 3 antibody, Adenylate cyclase antibody, Adenylate cyclase type 3 antibody, Adenylate cyclase type III antibody, Adenylyl cyclase 3 antibody, ATP pyrophosphate lyase antibody, ATP pyrophosphate-lyase 3 antibody, olfactive type antibody

UniProt Details

UniProt (Immunogen Species): P21932

NCBI Gene ID

64508

Product Specific Protocols

Western Blot Protocol: Download

General References

Fuchs, J.L. and Schwark, H.D., 2004. Neuronal primary cilia: a review. Cell Biology International, 28(2), pp.111-118. PMID: 14984756
Louvi, A. and Grove, E.A., 2011. Cilia in the CNS: the quiet organelle claims center stage. Neuron, 69(6), pp.1046-1060. PMID: 21435552

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Anti-Adenylate Cyclase III Antibody

Our Anti-Adenylate Cyclase III primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Adenylate Cyclase III and is antigen affinity purified. It is great for use in WB, IHC, ICC.