Anti-EB2 Antibody (KT52)

Name: Anti-EB2 Antibody
Brand: PhosphoSolutions
SKU: EM5081
Price: 360.0 USD
Availability: InStock

Our EB2 primary antibody from PhosphoSolutions is rat monoclonal. It detects human, mouse, and rat EB2 and is Protein G purified. It is great for use in WB, ICC.

Immunocytochemical labeling of EB2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-EB2 (EM5081) (right). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).

Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rat

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from PhosphoSolutions

SKU: EM5081

Volume: 100 µL

Variant

Price:

$360

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Product Details

Target

EB2

Target Description

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Format

Protein G Purified

Clonality

Monoclonal

Clone

KT52

Isotype

IgG2b

Applications

ICC, WB

Host Species

Rat

Gene Name

MAPRE2

Molecular Weight

Antigen

Clone KT52 was generated from a GST fusion protein containing human EB2. This sequence is highly conserved in rat and mouse EB2.

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.05% NaN3

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 36 kDa* protein corresponding to the molecular mass of EB2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB2 by immunocytochemistry in HeLa and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Microtubule RP/EB, MAPRE2, APC, EB2, End-binding

UniProt Details

UniProt (Human): Q15555
UniProt (Immunogen Species): Q15555

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Product Specific Protocols

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Anti-EB2 Antibody (KT52)

Our EB2 primary antibody from PhosphoSolutions is rat monoclonal. It detects human, mouse, and rat EB2 and is Protein G purified. It is great for use in WB, ICC.

SKU: EM5081

Product Details

Bulk Order Anti-EB2 Antibody

Product Specific Protocols

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