Antibody IgG

Antibody specificity is not as simple to understand as one might think. Epitopes, clonality, and purification technique all play a role in an antibody's specific interactions.

Contributed by Mike Browning

Specificity to a single epitope does not necessarily indicate specificity to a single protein.

It is common to see references to the “specificity” or “greater specificity” of monoclonal antibodies compared to polyclonal antibodies. Is that claim justified? No one would dispute the fact that a monoclonal is directed toward a single site or epitope of the target protein, while a polyclonal may contain antibodies directed toward multiple epitopes on the protein. However, in my opinion, that fact alone does not make monoclonal antibodies more specific. Indeed the single epitope toward which a monoclonal is directed may in fact be shared by many different proteins in addition to the protein of interest. Such a monoclonal would not be specific even though it recognizes only a single epitope. In contrast, a polyclonal antibody raised against the same protein may contain antibodies directed toward that same non-specific epitope as the monoclonal, as well as other epitopes that are more specific. In such a situation the serum of the polyclonal would at least contain some antibodies that are specific and thus it would be “more specific” than the monoclonal. Thus simply opting for a monoclonal is no guarantee of specificity and one must still utilize a full range of specificity controls.

Non-specific monoclonals may recognize multiple proteins. 

In figure 1 below, note that the monoclonal antibody recognizes the NET protein at ~50 kDa but it also recognizes proteins at 75 and 95 kDa.

non-specific monoclonal

Figure 1. The monoclonal raised against the NET protein labels three prominent bands at 50, 75 and 95 kD in a lysate of rat cortex.

Affinity purification can be used to isolate the most specific antibodies.

This may seem to some as a trivial issue but it can be extremely important in IHC where it is quite difficult to control for cross reactivity. There is a big difference in the purification/selection of monoclonal and polyclonal antibodies. The process of purification and selection of monoclonal antibodies rarely involves screening for specificity. Thus the hybridoma screens of hundreds or thousands of clones typically rely solely on the ability to recognize a target in a plate assay. There is typically no selection for affinity for the target and or specificity. This contrasts with polyclonal antibody affinity purification which, as its name suggests, can preferentially yield higher affinity and higher specificity antibodies. 

Specificity controls are important when using any antibody - monoclonal or polyclonal.

Taken together these comments are not at all meant to minimize the very real importance of monoclonal antibodies in clinical environments. Rather these comments are meant to underscore the importance of specificity controls in using all antibodies and to show that, in some cases, polyclonal antibodies may be more specific than monoclonal antibodies.

Further Reading

Antibodies That DO NOT WORK in Western Blots

Why Don’t More Researchers Validate Antibodies with WB Before Doing IHC?

Why Do So Many Researchers Prefer Polyclonal Antibodies?

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