1925-SYNP IHC copy

 

Antibodies that work are extremely valuable research tools.   However, the search for such antibodies is made quite difficult by two different problems.  The first problem, “the good antibody problem” is that production of a good antibody is a difficult, time consuming process; and many researchers and antibody companies do not possess either the patience or the skill necessary to make good antibodies.  The second problem, “the bad technique problem” is that a good antibody may not “work” in all of the various antibody-based assays. This may occur because the antibody cannot detect its target in a particular type of assay (e.g. the antibody target may become denatured in formalin fixation, thus formalin fixation is a bad technique for this antibody).  Alternatively, incorrect technique (AKA  bad technique) in an assay may interfere with the antibody binding to its target. 
            I have been working with antibodies for quite some time and I have had many opportunities to observe antibodies that do and do not work.  For my students who used antibodies that worked, the outcome was often the development of important new insight into a specific protein’s role in normal or disease function.  In contrast, months of frustration and false leads were usually all that resulted when my students used antibodies that did not work.   In this blog I will attempt to discuss how to address both the good antibody problem and the bad technique problem so that more antibodies will work. 
            I am hoping that this will become a collaborative project and I invite anyone who cares about antibodies to contribute to the discussion by offering tips, advice and/or topics for discussion.  I look forward to talking with you on this blog in the coming months. 
            My next topic will be on a bad technique problem and is titled “Antibodies and westerns blots, the case of the missing protein.” This is better known as what happens when you use a poor lysis buffer.
Summary
Antibodies that Work Begins
Article Name
Antibodies that Work Begins
Description
Antibodies are extremely valuable research tools, however producing an antibody that works can be very challenging. Both bad antibodies and bad antibody technique can lead to frustration for researchers.
Author
Mike Browning
Publisher Name
PhosphoSolutions
Publisher Logo

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