Affinity purified from pooled serum. Learn more.

XIRP1 (Ser295) Antibody

Catalog #: p2400-295 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
XIRP1
Molecular Reference:
~250 kDa and ~150 kDa
Cite This Antibody:
PhosphoSolutions Cat# p2400-295, RRID:AB_2492287
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of mouse XIRP1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser295 residue of XIRP1. (Chang et al., 2013).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10- mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

 

Product Specific Reference

Chang, Y. W., Chang, Y. T., Wang, Q., Lin, J. J. C., Chen, Y. J., & Chen, C. C. (2013). Quantitative phosphoproteomic study of pressure-overloaded mouse heart reveals dynamin-related protein 1 as a modulator of cardiac hypertrophy. Molecular & Cellular Proteomics, 12(11), 3094-3107.  PMID: 23882026

XIRP1 Ser295 Antibody
Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser295 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).