Affinity purified from pooled serum. Learn more.

XIRP1 (Ser295) Antibody

We are the original manufacturer of our XIRP1 (Ser295) rabbit polyclonal phosphospecific antibody. It is affinity purified from pooled serum, and great in WB.

Catalog #: p2400-295 Categories: , Datasheet:


  • SizePrice
Pooled Serum
Affinity Purified from Pooled Serum
Species Tested:
Expected Reactivity:
Human, Canine, Non-human primate, Rat
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
Molecular Weight:
~250 kDa and ~150 kDa
Cite This Antibody:
PhosphoSolutions Cat# p2400-295, RRID:AB_2492287
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of mouse XIRP1.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser295 residue of XIRP1. (Chang et al., 2013).

Synonyms: ExpandCollapse

• AI415219 antibody
• Cardiomyopathy-associated protein 1 antibody
• CMYA1 antibody
• DKFZp451D042 antibody
• DKFZp779C1255 antibody
• DKFZp779C1947 antibody
• MGC144145 antibody
• MGC144146 antibody
• Xin actin-binding repeat-containing protein 1 antibody
• XIN antibody
• Xirp1 antibody
• XIRP1_HUMAN antibody


100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.


Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID Dilution Publication
23882026 not listed Chang, Y.W., et al. 2013 Quantitative phosphoproteomic study of pressure-overloaded mouse heart reveals dynamin-related protein 1 as a modulator of cardiac hypertrophy. Molecular & Cellular Proteomics, 12(11), 3094-3107.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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