Vimentin Antibody, Chicken

Excellent cytoskeletal marker in developing neurons, astrocytes and fibroblasts! Our Vimentin chicken polyclonal antibody is formulated as total IgY fraction, and is excellent for use in WB, IHC, and ICC.

Catalog #: 2105-VIM Categories: , , , Datasheet:


  • SizePrice
Chicken, total IgY fraction
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Mammalian Tissues
WB 1:1000IHC 1:500-1:5000ICC 1:500-1:10,000 Don't see your application?
Host Species:
Chicken Polyclonal
Gene Name:
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2105-VIM, RRID:AB_2492283
Antigen/Purification: ExpandCollapse

The antigen is a recombinant human Vimentin purified from E. coli.

The antibody is Total IgY fraction.

Biological Significance: ExpandCollapse

Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Synonyms: ExpandCollapse

• CTRCT30 antibody
• Epididymis luminal protein 113 antibody
• FLJ36605 antibody
• HEL113 antibody
• VIM antibody
• VIME_HUMAN antibody
• Vimentin antibody


Liquid. Contains 10 mM sodium azide.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific Protocol


HeLa Cells.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: mouse
PMID Dilution Publication
26206560 not listed Kirane, A., et al. 2015. Warfarin blocks Gas6-mediated Axl activation required for pancreatic cancer epithelial plasticity and metastasis. Cancer Research, 75(18), pp.3699-3705.
25060520 not listed Ostapoff, K. T., et al. 2014. Neutralizing murine TGFβR2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis. Cancer Research, 74(18), 4996-5007.
23696215 not listed Ostapoff, K. T., et al. 2013. PG545, an angiogenesis and heparanase inhibitor, reduces primary tumor growth and metastasis in experimental pancreatic cancer. Molecular Cancer Therapeutics, 12(7), pp.1190-1201.
22829202 not listed Kirane, A., et al. 2012. Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer. Clinical Cancer Research, 18(18), pp.5031-5042
22678114 not listed Kirane, A., et al. 2012. Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib. Carcinogenesis, 33(9), 1639-46.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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