Labels A II amacrine cells in retina!
- Affinity Purified from Pooled Serum
- 100% Homology:
- Bovine, Canine, Chicken, Mouse, Non-human primate, Rat
- WB 1:1,000
- Rabbit polyclonal
- Gene Name:
- Molecular Reference:
- ~53 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 2100-VGAT, RRID:AB_2492282
The antigen is a peptide corresponding to amino acid residues from the N-terminal region of rat Vesicular GABA Amino Acid Transporter (VGAT).
The antibody is prepared from rabbit serum by affinity purification on a column made with the peptide used as antigen.
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.
Product Specific References
Santos, R.A., Fuertes, A.J., Short, G., Donohue, K.C., Shao, H., Quintanilla, J., Malakzadeh, P. and Cohen-Cory, S., 2018. DSCAM differentially modulates pre-and postsynaptic structural and functional central connectivity during visual system wiring. Neural development, 13(1), p.22. PMID: 30219101
Wang, X., Zorio, D.A., Schecterson, L., Lu, Y. and Wang, Y., 2018. Postsynaptic FMRP regulates synaptogenesis in vivo in the developing cochlear nucleus. Journal of Neuroscience, 38(29), pp.6445-6460. PMID: 29950504
Ka, M., Kook, Y.H., Liao, K., Buch, S. and Kim, W.Y., 2016. Transactivation of TrkB by Sigma-1 receptor mediates cocaine-induced changes in dendritic spine density and morphology in hippocampal and cortical neurons. Cell Death & Disease, 7(10), p.e2414. PMID: 27735948
Ka, M., Chopra, D. A., Dravid, S. M., & Kim, W. Y. (2016). Essential Roles for ARID1B in Dendritic Arborization and Spine Morphology of Developing Pyramidal Neurons. The Journal of Neuroscience, 36(9), 2723-2742. PMID: 26937011
Rotschafer, S. E., Marshak, S., & Cramer, K. S. (2015). Deletion of Fmr1 Alters Function and Synaptic Inputs in the Auditory Brainstem. PloS one, Feb 13;10(2):e0117266. PMID: 25679778
Noseda, R., Kainz, V., Borsook, D., & Burstein, R. (2014). Neurochemical pathways that converge on thalamic trigeminovascular neurons: potential substrate for modulation of migraine by sleep, food intake, stress and anxiety. PLoS One. Aug 4;9(8):e103929. PMID: 25090640