Affinity purified from pooled serum. Learn more.

Vesicular GABA Amino Acid Transporter (VGAT) Antibody

Catalog #: 2100-VGAT Categories: , , Datasheet:

$109.00$365.00

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Labels A II amacrine cells in retina!

 

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Chicken, Mouse, Rat, Xenopus
Species Reactivity:
Bovine, Canine, Non-human primate
Applications:
WB 1:1000IHC 1:100-1:1000ICC 1:500
Species:
Rabbit polyclonal
Gene Name:
SLC32A1
Molecular Weight:
~53 kDa
Cite This Antibody:
PhosphoSolutions Cat# 2100-VGAT, RRID:AB_2492282
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of rat Vesicular GABA Amino Acid Transporter (VGAT).

The antibody is prepared from rabbit serum by affinity purification on a column made with the peptide used as antigen.

Biological Significance: ExpandCollapse

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Synonyms: ExpandCollapse

• bA122O1.1 antibody
• GABA and glycine transporter antibody
• hVIAAT antibody
• SLC32A1 antibody
• SLC32A 1 antibody
• Slc32a1 antibody
• solute carrier family 32 (GABA vesicular transporter) member 1 antibody
• Solute carrier family 32 member 1 antibody
• Vesicular GABA Amino Acid Transporter antibody
• Vesicular GABA transporter antibody
• Vesicular inhibitory amino acid transporter antibody
• VGAT antibody
• VIAAT antibody
• VIAAT_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Immunohistochemistry

Rat Brain

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Chicken | Mouse | Rat
Immunohistochemistry: Frog | Mouse | Rat


Immunocytochemistry: Chicken
PMID Dilution Publication
29950504 1:500 Wang, X., Zorio, D.A.R., et al. 2018. Postsynaptic FMRP regulates synaptogenesis in vivo in the developing cochlear nucleus. Journal of Neuroscience, 38(29), pp.6445-6460.


Immunocytochemistry: Mouse
PMID Dilution Publication
26937011 not listed Ka, M., et al. 2016. Essential Roles for ARID1B in Dendritic Arborization and Spine Morphology of Developing Pyramidal Neurons. The Journal of Neuroscience, 36(9), 2723-2742.


Immunocytochemistry: Rat
PMID Dilution Publication
27735948 not listed Ka, M., et al. 2016. Transactivation of TrkB by Sigma-1 receptor mediates cocaine-induced changes in dendritic spine density and morphology in hippocampal and cortical neurons. Cell Death & Disease, 7(10), p.e2414.


Immunohistochemistry: Frog
PMID Dilution Publication
30219101 1:100 Santos, R.A., et al. 2018. DSCAM differentially modulates pre-and postsynaptic structural and functional central connectivity during visual system wiring. Neural development, 13(1), p.22.


Immunohistochemistry: Mouse
PMID Dilution Publication
25679778 1:200 Rotschafer, S.E., et al. 2015. Deletion of Fmr1 Alters Function and Synaptic Inputs in the Auditory Brainstem. PloS one, Feb 13;10(2):e0117266.


Immunohistochemistry: Rat
PMID Dilution Publication
25090640 1:1000 Noseda, R., et al. 2014. Neurochemical pathways that converge on thalamic trigeminovascular neurons: potential substrate for modulation of migraine by sleep, food intake, stress and anxiety. PLoS One. Aug 4;9(8):e103929.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Vesicular GABA Amino Acid Transporter (VGAT) Antibody
Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53 kDa VGAT protein.

Click here to view our Western blotting and lysate preparation protocols.

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